SpyCatcher-SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells.
SpyCatcher-SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells.
复制标题
SpyCatcher-SpyTag 用量子点介导子代杆状病毒的原位标记,用于追踪活细胞中的病毒感染。
DOI:
10.1039/c7cc08880a
复制
发表时间:
2018-01
期刊:
影响因子:
--
通讯作者:
Wang Hanzhong
中科院分区:
文献类型:
--
作者:
Ke Xianliang;Zhang Yuan;Zheng Fangliang;Liu Yan;Zheng Zhenhua;Xu Yi;Wang Hanzhong
A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a SpyTag into the viral glycoprotein, followed by specific conjugation with the SpyCatcher protein on modified quantum dots (QDs) through an isopeptide bond. The labelling method is convenient and efficient and shows little attenuation of viral infectivity. Therefore, it is a biologically compatible technique for tracking viral infection.
登录
查看更多内容
影响因子:
7.4
作者:
Huang, Li-Li;Lu, Gui-Hong;Xie, Hai-Yan
通讯作者:
Xie, Hai-Yan
影响因子:
14
作者:
Zhang, Fuxian;Zheng, Zhenhua;Wang, Hanzhong
通讯作者:
Wang, Hanzhong
影响因子:
3
作者:
Au, Shelly;Pante, Nelly
通讯作者:
Pante, Nelly
影响因子:
4
作者:
Huang, Bi-Hai;Lin, Yi;Pang, Dai-Wen
通讯作者:
Pang, Dai-Wen
DOI:
10.1146/annurev-anchem-060908-155136
发表时间:
2013
期刊:
Annual review of analytical chemistry (Palo Alto, Calif.)
影响因子:
--
作者:
Kairdolf BA;Smith AM;Stokes TH;Wang MD;Young AN;Nie S
通讯作者:
Nie S