Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.).
Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.).
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DOI：
10.1093/jxb/erp289
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发表时间：
2010
影响因子：
6.9
通讯作者：
Craufurd PQ
中科院分区：
文献类型：
--
作者：
Jagadish SV;Muthurajan R;Oane R;Wheeler TR;Heuer S;Bennett J;Craufurd PQ
水稻开花期高温是水稻对温度最敏感的时期，在未来的气候条件下，高温的发生将更加频繁。以3个水稻品种为材料，研究了开花期高温对生殖器官形态、花粉数量和花药蛋白表达的影响。基因型在开花期将植物暴露于6小时高温（38 °C）和对照温度（29 °C），并收集小穗用于形态学和蛋白质组学分析。Moroberekan是最热敏感的基因型（38 °C下小穗育性为18%），而IR 64（48%）和N22（71%）分别是中度和高度耐热的。花药长、宽、顶孔长、基孔长、顶孔面积、柱头长、雌蕊长在基因型间存在显著差异。温度也影响了这些性状，增加花药孔径和减少柱头长度。尽管如此，柱头上的花粉数量的变化不能与测量的形态特征。小穗育性变异与柱头上萌发花粉粒数≥20粒的小穗比例呈高度相关（r=0.97，n=6）。双向凝胶电泳显示46个蛋白质点的丰度发生了变化，其中13个差异表达蛋白质点进行了MS/MALDI-TOF分析。发现N22中的冷休克蛋白和热休克蛋白显著上调，这可能有助于N22更大的耐热性。讨论了花药开裂和授粉过程中差异表达蛋白和形态在形成耐热性和敏感性中的作用。
Episodes of high temperature at anthesis, which in rice is the most sensitive stage to temperature, are expected to occur more frequently in future climates. The morphology of the reproductive organs and pollen number, and changes in anther protein expression, were studied in response to high temperature at anthesis in three rice (Oryza sativa L.) genotypes. Plants were exposed to 6 h of high (38 °C) and control (29 °C) temperature at anthesis and spikelets collected for morphological and proteomic analysis. Moroberekan was the most heat-sensitive genotype (18% spikelet fertility at 38 °C), while IR64 (48%) and N22 (71%) were moderately and highly heat tolerant, respectively. There were significant differences among the genotypes in anther length and width, apical and basal pore lengths, apical pore area, and stigma and pistil length. Temperature also affected some of these traits, increasing anther pore size and reducing stigma length. Nonetheless, variation in the number of pollen on the stigma could not be related to measured morphological traits. Variation in spikelet fertility was highly correlated (r=0.97, n=6) with the proportion of spikelets with ≥20 germinated pollen grains on the stigma. A 2D-gel electrophoresis showed 46 protein spots changing in abundance, of which 13 differentially expressed protein spots were analysed by MS/MALDI-TOF. A cold and a heat shock protein were found significantly up-regulated in N22, and this may have contributed to the greater heat tolerance of N22. The role of differentially expressed proteins and morphology during anther dehiscence and pollination in shaping heat tolerance and susceptibility is discussed.
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DOI：
10.1093/dnares/1.1.15
发表时间：
1994-01-01
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Kobayashi, T;Kobayashi, E;Tabata, S
通讯作者：
Tabata, S
影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
影响因子：
5.8
作者：
Craufurd, PQ;Prasad, PVV;Nigam, SN
通讯作者：
Nigam, SN
DOI：
10.1007/bf02488823
发表时间：
1986-06-01
期刊：
BOTANICAL MAGAZINE-TOKYO
影响因子：
--
作者：
BABBAR, SB;GUPTA, SC
通讯作者：
GUPTA, SC
影响因子：
7.4
作者：
Honys, D;Twell, D
通讯作者：
Twell, D