Molecular beacon based real-time PCR p1 gene genotyping, macrolide resistance mutation detection and clinical characteristics analysis of Mycoplasma pneumoniae infections in children.
Molecular beacon based real-time PCR p1 gene genotyping, macrolide resistance mutation detection and clinical characteristics analysis of Mycoplasma pneumoniae infections in children.
复制标题
基于分子信标的儿童肺炎支原体感染p1基因基因分型、大环内酯类耐药突变检测及临床特征分析
DOI：
10.1186/s12879-022-07715-6
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发表时间：
2022-09-06
影响因子：
3.7
通讯作者：
Zhang, Wancun
中科院分区：
文献类型：
--
作者：
Li, Lifeng;Ma, Jiayue;Guo, Pengbo;Song, Xiaorui;Li, Mingchao;Yu, Zengyuan;Yu, Zhidan;Cheng, Ping;Sun, Huiqing;Zhang, Wancun
根据粘附蛋白P1的序列差异可将肺炎支原体分为不同的亚型，但不同亚型与大环内酯类药物耐药及临床表现的关系尚不清楚。本研究建立了基于分子信标的实时聚合酶链反应（real-time PCR）p1基因分型方法，分析了大环内酯类耐药基因突变及其与临床特征的关系。建立了基于分子信标的p1基因实时荧光定量PCR分型方法，通过PCR和测序分析大环内酯类药物耐药基因突变位点，并分析其与临床特征的关系。检测限为1 - 100个拷贝/反应。两种亚型间无交叉反应。总共，来自100例M.使用基于信标的实时PCR方法和P1 - 1 M对2019年和2021年肺炎检测结果进行基因分型。肺炎占69.0%。所有患者均存在大环内酯类耐药相关的23SrRNA基因A2063G突变。同时发现了新的突变，分别为C2622T、C2150A、C2202G和C2443A突变。p1基因分型与临床特征的关系无统计学意义。建立了一种快速、简便的p1基因分子信标实时荧光PCR分型方法。从1型转变为2型，大环内酯类耐药率为100.0%。本研究为M.为控制大环内酯类抗生素高耐药提供有价值的流行病学监测信息和临床治疗指导。在线版本包含补充材料，可通过10.1186/s12879 - 022 - 07715 - 6获得。
Mycoplasma pneumoniae can be divided into different subtypes on the basis of the sequence differences of adhesive protein P1, but the relationship between different subtypes, macrolide resistance and clinical manifestations are still unclear. In the present study, we established a molecular beacon based real-time polymerase chain reaction (real-time PCR) p1 gene genotyping method, analyzed the macrolide resistance gene mutations and the relationship of clinical characteristics with the genotypes. A molecular beacon based real-time PCR p1 gene genotyping method was established, the mutation sites of macrolide resistance genes were analyzed by PCR and sequenced, and the relationship of clinical characteristics with the genotypes was analyzed. The detection limit was 1–100 copies/reaction. No cross-reactivity was observed in the two subtypes. In total, samples from 100 patients with positive M. pneumoniae detection results in 2019 and 2021 were genotyped using the beacon based real-time PCR method and P1-1 M. pneumoniae accounted for 69.0%. All the patients had the A2063G mutation in the macrolide resistance related 23S rRNA gene. Novel mutations were also found, which were C2622T, C2150A, C2202G and C2443A mutations. The relationship between p1 gene genotyping and the clinical characteristics were not statistically related. A rapid and easy clinical application molecular beacon based real-time PCR genotyping method targeting the p1 gene was established. A shift from type 1 to type 2 was found and 100.0% macrolide resistance was detected. Our study provided an efficient method for genotyping M. pneumoniae, valuable epidemiological monitoring information and clinical treatment guidance to control high macrolide resistance. The online version contains supplementary material available at 10.1186/s12879-022-07715-6.
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影响因子：
9.4
作者：
SU, CJ;DALLO, SF;BASEMAN, JB
通讯作者：
BASEMAN, JB
DOI：
10.1007/bf01586183
发表时间：
1996-01-01
影响因子：
4.5
作者：
Jacobs, E;Vonski, M;Pietsch, K
通讯作者：
Pietsch, K
DOI：
10.3390/antibiotics10101216
发表时间：
2021-10-07
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Pereyre S;Tardy F
通讯作者：
Tardy F
DOI：
10.3390/pathogens10020119
发表时间：
2021-01-25
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Jiang Z;Li S;Zhu C;Zhou R;Leung PHM
通讯作者：
Leung PHM
影响因子：
1.9
作者：
Feeney M;Murphy K;Lopilato J
通讯作者：
Lopilato J