Analysis of the Metabolites of Indole Degraded by an Isolated Acinetobacter pittii L1.
Analysis of the Metabolites of Indole Degraded by an Isolated Acinetobacter pittii L1.
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DOI：
10.1155/2017/2564363
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发表时间：
2017
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Yang Z;Zhou J;Xu Y;Zhang Y;Luo H;Chang K;Wang Y
吲哚及其衍生物是典型的氮杂环化合物，因其对人类和生态环境具有毒性、顽固性和致癌性而备受关注。本研究从焦化废水中分离出一株革兰氏阴性菌。经生理生化鉴定和16S核糖体DNA （rDNA）基因序列同源性分析，证实该菌株为pittii不动杆菌L1。在37℃、pH 7.4、150 rpm的最佳条件下，菌株可在48 h内完全去除400 mg/L吲哚。在吲哚生物降解过程中，有机氮转化为NH3-N，再转化为NO3 -，有机碳部分转化为CO2。通过液相色谱串联质谱（LC-MS/MS）对靛蓝、4-（3-羟基- 1h -吡咯-2-基）-2-氧-丁-3-烯酸和isatin的分析，提出了吲哚降解的代谢途径。利用Microtox试验对生物降解产物的毒性进行了评估，结果表明代谢物的毒性大于吲哚。我们的研究为pittii不动杆菌L1在NHCs降解、靛蓝生产、土壤修复以及含吲哚废水处理方面的潜在应用提供了希望。
Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human and ecological environment. In this study, a Gram-negative bacterial strain of eliminating indole was isolated from a coking wastewater. The strain was confirmed as Acinetobacter pittii L1 based on the physiological and biochemical characterization and 16S ribosomal DNA (rDNA) gene sequence homology. 400 mg/L indole could be completely removed within 48 h by the strain on the optimum condition of 37°C, pH 7.4, and 150 rpm. The organic nitrogen was converted to NH3-N and then to NO3− and the organic carbon was partially transferred to CO2 during the indole biodegradation. The metabolic pathways were proposed to explain the indole degradation based on the liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of indigo, 4-(3-Hydroxy-1H-pyrrol-2-yl)-2-oxo-but-3-enoic acid, and isatin. The toxicity of the biodegradation products was evaluated using the Microtox test, which revealed that the metabolites were more toxic than indole. Our research holds promise for the potential application of Acinetobacter pittii L1 for NHCs degradation, production of indigoids, and soil remediation as well as treatment of indole containing wastewater.
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影响因子：
3
作者：
Wang, Jing;Zhang, Xuwang;Peng, Xiaojun
通讯作者：
Peng, Xiaojun
DOI：
10.1073/pnas.260499897
发表时间：
2000-12-19
影响因子：
11.1
作者：
Frey, M;Stettner, C;Gierl, A
通讯作者：
Gierl, A
影响因子：
2
作者：
Zou, Peng;Koh, Hwee Ling
通讯作者：
Koh, Hwee Ling
影响因子：
11.4
作者：
He, Sin-Yi;Lin, Yun-Huin;Hwang, Sz-Chwun John
通讯作者：
Hwang, Sz-Chwun John
DOI：
10.1016/j.ibiod.2005.10.001
发表时间：
2005-12-01
影响因子：
4.8
作者：
Yin, B;Gu, JD;Wan, NS
通讯作者：
Wan, NS