Human amniotic epithelial cells differentiate into cells expressing germ cell specific markers when cultured in medium containing serum substitute supplement.
Human amniotic epithelial cells differentiate into cells expressing germ cell specific markers when cultured in medium containing serum substitute supplement.
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当在含有血清替代补充剂的培养基中培养时，人羊膜上皮细胞分化为表达生殖细胞特异性标记的细胞。
DOI：
10.1186/1477-7827-10-108
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发表时间：
2012-12-14
期刊：
影响因子：
--
通讯作者：
Shalev E
中科院分区：
文献类型：
--
作者：
Evron A;Goldman S;Shalev E
人羊膜上皮细胞 (hAEC) 维持原肠胚形成前胚胎细胞的可塑性，具有分化为所有三个胚层的潜力。以前从未描述过这些细胞分化成表达生殖细胞特异性标记物的细胞的潜力。在本研究中，hAEC 在含有血清替代补充剂 (SSS) 的培养基中培养。通过逆转录聚合酶链式反应（RT-PCR）、免疫荧光染色和流式激活细胞分选分析（FACS），在分化为表达生殖细胞特异性标记的细胞的不同时间点评估hAEC中生殖细胞和卵母细胞特异性标记的基因和蛋白表达。当用 SSS 培养时，在第 1 代，hAEC 开始表达生殖细胞特异性基因 C-KIT、DAZL、VASA 和 ZP3，并在第 5 代出现类似卵母细胞的大圆形细胞。这些细胞在蛋白质水平上表达生殖细胞特异性标记物 DAZL、卵母细胞特异性标记物 GDF9 和 ZP3 以及减数分裂特异性标记物 DMC1 和 SCP3。根据我们的初步结果，我们可以得出结论，hAEC 具有分化为表达生殖细胞特异性标记物的细胞的潜力。
Human amniotic epithelial cells (hAECs) maintain the plasticity of pregastrulation embryonic cells, having the potential to differentiate into all three germ layers. The potential of these cells to differentiate into cells expressing germ cell specific markers has never been described before. In the present study, hAECs were cultured in medium containing serum substitute supplement (SSS). Gene and protein expression of germ cell and oocyte specific markers was assessed by reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence staining and flow activated cell sorter analysis (FACS) in hAECs at different time points during the differentiation into cells expressing germ cell specific markers. When cultured with SSS, already at passage 1, hAECs start to express the germ cell specific genes C-KIT, DAZL, VASA and ZP3 and at passage 5 large round cells, resembling oocytes, appeared. The cells express the germ cell specific marker DAZL, the oocyte specific markers GDF9 and ZP3 and the meiosis specific markers DMC1 and SCP3 at the protein level. From our preliminary results we can conclude that hAECs have the potential to differentiate into cells expressing germ cell specific markers.
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影响因子：
2.8
作者：
de Matos, DG;Furnus, CC
通讯作者：
Furnus, CC
影响因子：
6.1
作者：
Meintjes, Marius;Chantilis, Samuel J.;Madden, James D.
通讯作者：
Madden, James D.
影响因子：
5.2
作者：
Eguizabal, C.;Montserrat, N.;Belmonte, J. C. Izpisua
通讯作者：
Belmonte, J. C. Izpisua
影响因子：
--
作者：
Miki, Toshio;Marongiu, Fabio;C Strom, Stephen
通讯作者：
C Strom, Stephen
影响因子：
4
作者：
Danner, S.;Kajahn, J.;Kruse, C.
通讯作者：
Kruse, C.