Antibacterial Properties of Canine Platelet-Rich Plasma and Other Non-Transfusional Hemo-Components: An in vitro Study.
Antibacterial Properties of Canine Platelet-Rich Plasma and Other Non-Transfusional Hemo-Components: An in vitro Study.
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犬血小板血浆和其他非转移血液兼容性的抗菌特性：一项体外研究。
DOI：
10.3389/fvets.2021.746809
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发表时间：
2021
影响因子：
3.2
通讯作者：
Tambella AM
中科院分区：
文献类型：
--
作者：
Attili AR;Iacoucci C;Serri E;Cuteri V;Cantalamessa A;Linardi M;Rifici C;Mazzullo G;Rossi G;Galosi L;Tambella AM
本体外实验旨在评价犬非输血血液成分的潜在抗菌性能。通常用于再生医学目的的治疗制剂（富血小板血浆、血小板凝胶、血小板裂解液、纤维蛋白胶），考虑到白细胞丰富和白细胞贫乏的制剂，以及血小板贫乏的血浆和激活物质（凝血酶、葡萄糖酸钙），进行了检测，以检测具有潜在抗菌特性的元素。对从犬伤口分离的不同菌株（金黄色葡萄球菌亚种、柯氏葡萄球菌亚种、大肠杆菌、铜绿假单胞菌和肺炎克雷伯菌亚种）进行抗菌效果测试，并对已知的人类和兽医抗生素分类为敏感菌、多药耐药菌、广泛耐药菌和普遍耐药菌。采用琼脂扩散法（Kirby-Bauer）和微抑制法（微孔板法和分光光度计法）进行评价。研究结果证实了犬非输血血液成分的抗菌特性。发现对革兰氏阴性菌具有更有效的抑菌作用，也具有耐药性。白细胞或血小板的存在似乎并不是抗菌作用的必要条件。抗菌活性的确切作用机制有待进一步研究。然而，非输血血液成分可能是一种有用的自然援助，以控制狗的细菌感染。
This in vitro study was carried out to evaluate the potential antibacterial properties of canine non-transfusional hemo-components. Therapeutic formulations commonly used for regenerative medicine purposes (platelet-rich plasma, platelet gel, platelet lysate, fibrin glue), considering both leukocyte-rich and leukocyte-poor formulations, but also platelet-poor plasma and activating substances (thrombin, calcium gluconate), were tested to detect elements with potential antimicrobial properties. The antibacterial effect was tested on different bacterial strains (Staphylococcus aureus subspecies aureus, Staphylococcus cohnii subspecies cohnii, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae subspecies pneumoniae) isolated from canine wounds and classified as susceptible, multidrug-, extensively, and pandrug-resistant bacteria toward a known panel of human and veterinary antibiotics. The evaluation was carried out by agar gel diffusion method (Kirby–Bauer) and micro-inhibition in broth using microplates and spectrophotometer reading. The study findings confirmed the hypothesized antibacterial properties of canine non-transfusional hemo-components. A more effective bacteriostatic effect was found against Gram-negative bacteria, drug-resistant too. The presence of leukocytes or platelets does not appear to be essential for the antibacterial effect. Further studies should be conducted to evaluate the exact mechanism of action of the antimicrobial activity. However, non-transfusional hemo-components could be a useful natural aid in controlling bacterial infections in dogs.
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DOI：
10.1002/jor.24584
发表时间：
2020-06
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Gilbertie JM;Schaer TP;Schubert AG;Jacob ME;Menegatti S;Ashton Lavoie R;Schnabel LV
通讯作者：
Schnabel LV
DOI：
10.1007/s00068-018-0957-0
发表时间：
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影响因子：
2.1
作者：
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通讯作者：
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DOI：
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发表时间：
2016-05-01
期刊：
Indian journal of dental research : official publication of Indian Society for Dental Research
影响因子：
--
作者：
Badade, Pallavi S;Mahale, Swapna A;Warang, Ayushya D
通讯作者：
Warang, Ayushya D
影响因子：
1.9
作者：
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通讯作者：
Andia, Isabel
DOI：
10.2460/javma.243.9.1291
发表时间：
2013-11-01
影响因子：
1.9
作者：
Fahie, Maria A.;Ortolano, Girolamo A.;Bertone, Alicia L.
通讯作者：
Bertone, Alicia L.