Sperm quality and absence of SARS-CoV-2 RNA in semen after COVID-19 infection: a prospective, observational study and validation of the SpermCOVID test.
Sperm quality and absence of SARS-CoV-2 RNA in semen after COVID-19 infection: a prospective, observational study and validation of the SpermCOVID test.
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DOI：
10.1016/j.fertnstert.2021.10.022
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发表时间：
2022-03
影响因子：
6.7
通讯作者：
Depuydt CE
中科院分区：
文献类型：
--
作者：
Donders GGG;Bosmans E;Reumers J;Donders F;Jonckheere J;Salembier G;Stern N;Jacquemyn Y;Ombelet W;Depuydt CE
目的探讨新冠肺炎感染康复后精子的传染性及其对生育能力的影响。前瞻性队列研究。大学医疗中心。120名已从证实的新冠肺炎感染中康复的比利时男子。未进行任何干预。精液质量使用世界卫生组织的标准进行评估。通过DNA断裂指数和高密度染色来评价精子细胞的DNA损伤。抗SARS-CoV2刺突-1抗原抗体、核抗体和S1受体结合区抗体分别用ELISA法和化学发光微粒子免疫法检测。在感染后不久和以后的一段时间内，在精液中都没有检测到SARS-CoV-2RNA。60%的受试者在新冠肺炎感染后不久(1个月)，37%的受试者在新冠肺炎感染后1-2个月，28%的受试者在新冠肺炎感染后2个月，受试者的平均进行性运动能力下降。在新冠肺炎感染后不久(1个月)进行检测的男性中，37%的人平均精子数量减少，29%的男性在新冠肺炎感染后1-2个月检测，6%的男性在新冠肺炎感染2个月后检测。新冠肺炎感染的严重程度和发热与精子特征无关，但精子畸形与血清抗SARS-CoV-2Ig G抗体效价和SPAK1受体结合区有较强的相关性，而与核苷酸无相关性。3名男性(2.5%)出现高水平的抗精子抗体。精液在新冠肺炎感染后1周或以上(平均53天)未感染SARS-CoV-2。然而，想要怀孕的夫妇应该得到警告，感染新冠肺炎后的精子质量可能会低于最佳水平。估计恢复时间为3个月，但进一步的后续研究正在进行中，以证实这一点，并确定是否在少数男性中发生了永久性损害。
To study the contagiousness of sperm and its influence on fertility after recovery from COVID-19 infection. Prospective cohort study. University medical center. One hundred twenty Belgian men who had recovered from proven COVID-19 infection. No intervention was performed. Semen quality was assessed using the World Health Organisation criteria. DNA damage to sperm cells was assessed by quantifying the DNA fragmentation index and the high density stainability. Finally antibodies against SARS-CoV2 spike-1 antigen, nuclear and S1-receptor binding domain were measured by Elisa and chemilumenscent microparticle immunoassays, respectively. SARS-CoV-2 RNA was not detected in semen during the period shortly after infection nor at a later time. Mean progressive motility was reduced in 60% of men tested shortly (<1 month) after COVID-19 infection, 37% of men tested 1 to 2 months after COVID-19 infection, and 28% of men tested >2 months after COVID-19 infection. Mean sperm count was reduced in 37% of men tested shortly (<1 month) after COVID-19 infection, 29% of men tested 1 to 2 months after COVID-19 infection, and 6% of men tested >2 months after COVID-19 infection. The severity of COVID-19 infection and the presence of fever were not correlated with sperm characteristics, but there were strong correlations between sperm abnormalities and the titers of SARS-CoV-2 IgG antibody against spike 1 and the receptor- binding domain of spike 1, but not against nucleotide, in serum. High levels of antisperm antibodies developed in three men (2.5%). Semen is not infectious with SARS-CoV-2 at 1 week or more after COVID-19 infection (mean, 53 days). However, couples with a desire for pregnancy should be warned that sperm quality after COVID-19 infection can be suboptimal. The estimated recovery time is 3 months, but further follow-up studies are under way to confirm this and to determine if permanent damage occurred in a minority of men.
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