The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters.
The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters.
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野油菜黄单胞菌的 Zur 通过识别其靶启动子内的两个不同序列，充当假定的锌稳态基因的阻遏子和激活子
DOI：
10.1093/nar/gkn328
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发表时间：
2008-08
影响因子：
14.9
通讯作者：
Tang, Ji-Liang
中科院分区：
文献类型：
--
作者：
Huang, Dong-Liang;Tang, Dong-Jie;Liao, Qing;Li, Heng-Cong;Chen, Qi;He, Yong-Qiang;Feng, Jia-Xun;Jiang, Bo-Le;Lu, Guang-Tao;Chen, Baoshan;Tang, Ji-Liang
长期以来，人们一直认为细菌中锌的稳态是由输出系统和摄取系统维持的，这两个系统分别由其自身的调节因子控制，而摄取系统由Zur负调节，Zur结合存在于其靶启动子中的约30-bp的富含AT的序列，称为转录盒，以阻断RNA聚合酶的进入。在这里，我们证明了在体内和体外，除了作为一个阻遏物的推定的锌吸收系统，Zur的细菌性植物病原体野油菜黄单胞菌pathovar野油菜（XCC）作为一个激活剂的锌外排泵。Xcc Zur结合到一个类似的双链盒，在编码推定的Zn 2 +-摄取系统的基因的启动子中具有30-bp的富含AT的序列，但在编码Zn 2 +-输出系统的基因的启动子的−35至−10序列上具有59-bp的富含GC的序列，该序列具有20-bp的反向重复序列。反向重复序列的突变导致Zur的体外结合和输出基因的启动子的体内和体外激活的废除。这些结果表明，Xcc Zur功能作为一个阻遏物和激活剂的推定锌稳态基因通过识别两个不同的序列在其目标启动子。
It has been long considered that zinc homeostasis in bacteria is maintained by export systems and uptake systems, which are separately controlled by their own regulators and the uptake systems are negatively regulated by Zur which binds to an about 30-bp AT-rich sequence known as Zur-box present in its target promoters to block the entry of RNA polymerase. Here, we demonstrated in vivo and in vitro that in addition to act as a repressor of putative Zn2+-uptake systems, the Zur of the bacterial phytopathogen Xanthomonas campestris pathovar campestris (Xcc) acts as an activator of a Zn2+ efflux pump. The Xcc Zur binds to a similar Zur-box with ∼30-bp AT-rich sequence in the promoters of the genes encoding putative Zn2+-uptake systems but a 59-bp GC-rich sequence with a 20-bp inverted repeat overlapping the promoter's −35 to −10 sequence of the gene encoding a Zn2+-export system. Mutagenesis of the inverted repeat sequence resulted in abolishment of the in vitro binding and the in vivo and in vitro activation of the export gene's promoter by Zur. These results reveal that the Xcc Zur functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters.
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影响因子：
3.6
作者：
Brocklehurst, KR;Hobman, JL;Morby, AP
通讯作者：
Morby, AP
影响因子：
2.1
作者：
Garrido, ME;Bosch, M;Barbé, J
通讯作者：
Barbé, J
影响因子：
4.8
作者：
Friedman, YE;O'Brian, MR
通讯作者：
O'Brian, MR
比较和功能基因组学揭示了植物病原体黄单胞菌参考菌株和大量中国分离株之间的遗传多样性和宿主特异性的决定因素。油菜。
影响因子：
12.3
作者：
He, Yong-Qiang;Zhang, Liang;Jiang, Bo-Le;Zhang, Zheng-Chun;Xu, Rong-Qi;Tang, Dong-Jie;Qin, Jing;Jiang, Wei;Zhang, Xia;Liao, Jie;Cao, Jin-Ru;Zhang, Sui-Sheng;Wei, Mei-Liang;Liang, Xiao-Xia;Lu, Guang-Tao;Feng, Jia-Xun;Chen, Baoshan;Cheng, Jing;Tang, Ji-Liang
通讯作者：
Tang, Ji-Liang
影响因子：
3.2
作者：
Anton, A;Grosse, C;Nies, DH
通讯作者：
Nies, DH