Challenges and opportunities of airborne metagenomics.
Challenges and opportunities of airborne metagenomics.
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DOI：
10.1093/gbe/evv064
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发表时间：
2015-05-06
影响因子：
3.3
通讯作者：
Mineta K
中科院分区：
文献类型：
--
作者：
Behzad H;Gojobori T;Mineta K
最近对海洋和土壤等环境的宏基因组研究，极大地增强了我们对生活在这些栖息地中的各种微生物群落及其在维持庞大生态系统中的重要作用的理解。与土壤和海洋宏基因组学相关的出版物数量的增加与空气的出版物数量形成鲜明对比，然而，空气中的微生物被认为对我们生活的许多方面产生重大影响，从它们在云形成、降水和大气化学等大气事件中的潜在作用到它们对人类健康的重大影响。在这篇综述中，我们将讨论当前航空宏基因组学的进展，并特别关注探索开展此类研究的挑战和机遇。开展空气微生物宏基因组研究的主要挑战如下：1)空气中微生物密度低；2)从空气中有效地检索微生物；3)空气中微生物群落组成的可变性；4)缺乏标准化的方案和方法；5)DNA测序和生物信息学相关的挑战。克服这些挑战可以为全面分析空气中微生物及其对大气、全球气候和我们健康的潜在影响奠定基础。宏基因组研究提供了一个独特的机会来检查空气中的病毒和细菌多样性，并监测它们在当地或全球的传播，包括病原微生物的威胁。航空宏基因组研究还可以发现与气象和工业应用、环境生物修复和生物地球化学循环相关的新基因和代谢途径。
Recent metagenomic studies of environments, such as marine and soil, have significantly enhanced our understanding of the diverse microbial communities living in these habitats and their essential roles in sustaining vast ecosystems. The increase in the number of publications related to soil and marine metagenomics is in sharp contrast to those of air, yet airborne microbes are thought to have significant impacts on many aspects of our lives from their potential roles in atmospheric events such as cloud formation, precipitation, and atmospheric chemistry to their major impact on human health. In this review, we will discuss the current progress in airborne metagenomics, with a special focus on exploring the challenges and opportunities of undertaking such studies. The main challenges of conducting metagenomic studies of airborne microbes are as follows: 1) Low density of microorganisms in the air, 2) efficient retrieval of microorganisms from the air, 3) variability in airborne microbial community composition, 4) the lack of standardized protocols and methodologies, and 5) DNA sequencing and bioinformatics-related challenges. Overcoming these challenges could provide the groundwork for comprehensive analysis of airborne microbes and their potential impact on the atmosphere, global climate, and our health. Metagenomic studies offer a unique opportunity to examine viral and bacterial diversity in the air and monitor their spread locally or across the globe, including threats from pathogenic microorganisms. Airborne metagenomic studies could also lead to discoveries of novel genes and metabolic pathways relevant to meteorological and industrial applications, environmental bioremediation, and biogeochemical cycles.
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影响因子：
3.6
作者：
Be, Nicholas A.;Thissen, James B.;Fofanov, Viacheslav Y.;Allen, Jonathan E.;Rojas, Mark;Golovko, George;Fofanov, Yuriy;Koshinsky, Heather;Jaing, Crystal J.
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DOI：
10.1073/pnas.0608255104
发表时间：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
4.2
作者：
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影响因子：
5
作者：
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通讯作者：
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