ACUTE MYELOGENOUS LEUKAEMIA WITH C-MYC AMPLIFICATION AND DOUBLE MINUTE CHROMOSOMES-猫眼课题宝

具有 C-MYC 扩增和双分钟染色体的急性髓性白血病

DOI：

10.1016/s0140-6736(85)90907-9

发表时间：

1985

期刊：

The Lancet

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

K. Alitalo;R. Winqvist;J. Keski‐oja;M. Ilvonen;K. Saksela;R. Alitalo;M. Laiho;S. Knuutila;A. Chapelle

对患有白血病前期的女性的骨髓细胞进行的细胞遗传学分析显示，存在大量具有 4 三体和双小染色体的有丝分裂。这些异常后来在随后的急性髓系白血病（AML）期间的血细胞中被发现。经过三个疗程的阿霉素、阿糖胞苷和泼尼松后，病情完全缓解。第一次复发后，在白血病细胞中发现了进一步的克隆异常，即 6 三体性。对复发期间外周血细胞总 DNA 的分析表明，c-myconcogene 在白血病细胞中扩增了约 30 倍。 N-myc、c-mos 和 c-myboncogenes 仅显示单拷贝信号。平均每个 dmin 染色体上大约有两个 c-mycreside 拷贝。在含有双小染色体的新鲜 AML 细胞中发现细胞癌基因 (c-myc) 扩增，表明某些白血病细胞群的克隆进化可能涉及选择增加癌基因剂量。

Cytogenetic analysis of bone-marrow cells from a woman with preleukaemia showed numerous mitoses with trisomy 4 and double minute chromosomes. These abnormalities were later seen in blood cells during subsequent acute myeloid leukaemia (AML). Complete remission was achieved with three courses of doxorubicin, cytosine arabinoside, and prednisone. A further clonal abnormality, trisomy 6, was seen in leukaemic cells after the first relapse. Analyses of total DNA from the peripheral-blood cells during relapse showed that the c-myconcogene was amplified about 30-fold in the leukaemic cells. The N-myc, c-mos, and c-myboncogenes showed only single-copy signals. On average about two copies of c-mycresided on each dmin chromosome. The finding of amplification of a cellular oncogene (c-myc) in fresh AML cells containing double minute chromosomes suggests that clonal evolution of some leukaemia cell populations may involve selection for increased dosage of oncogenes.