Alteration of selective neurotransmitters in fetal brains of prenatally alcohol-treated C57BL/6 mice: quantitative analysis using liquid chromatography/tandem mass spectrometry.
Alteration of selective neurotransmitters in fetal brains of prenatally alcohol-treated C57BL/6 mice: quantitative analysis using liquid chromatography/tandem mass spectrometry.
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DOI：
10.1016/j.ijdevneu.2010.01.004
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发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Mechref Y
中科院分区：
文献类型：
--
作者：
Sari Y;Hammad LA;Saleh MM;Rebec GV;Mechref Y
我们之前证明，产前酒精暴露会导致不同胚胎阶段的大脑缺陷。本研究旨在表征产前酒精暴露对早期胚胎 13 阶段 (E13) 几种神经递质水平的影响。从 E7 到 E13，怀孕的 C57BL/6 小鼠接受 25% 乙醇衍生热量饮食 (ALC) 或配对喂养 (PF) 液体饮食。在 E13 时，从 ALC 和 PF 组的母鼠身上采集胎儿大脑。然后使用液相色谱/串联质谱（LC-MS）来评估神经递质水平。该方法涉及使用液相色谱柱与多反应监测质谱联用。对儿茶酚胺、偶像胺和氨基酸神经递质的定量分析显示，与 PF 组相比，ALC 组的多巴胺 (p=0.004)、去甲肾上腺素 (p=0.0009)、肾上腺素 (p=0.0002)、血清素 (p=0.004) 和 GABA (p=0.002) 水平显着降低。然而，E13胎儿大脑中谷氨酸水平没有显着变化。这些发现表明，产前接触酒精会降低 E13 胎儿大脑中一些儿茶酚胺、偶像胺和氨基酸神经递质的浓度。这项研究表明，选择性神经递质的改变可能是胎儿酒精谱系障碍中大脑功能和行为异常的原因。
We previously demonstrated that prenatal alcohol exposure results in brain defects at different embryonic stages. This study is aimed at characterizing the influence of prenatal alcohol exposure on the levels of several neurotransmitters at early embryonic stage 13 (E13). Pregnant C57BL/6 mice were exposed to either a 25% ethanol derived calorie diet (ALC) or pair-fed (PF) liquid diet from E7 to E13. At E13, fetal brains were collected from dams of the ALC and PF groups. Liquid chromatography/tandem mass spectrometry (LC-MS) was then used to evaluate neurotransmitter levels. This approach involved the use of an LC column in conjunction with multiple-reaction monitoring mass spectrometry. Quantitative analyses of catecholamines, idolamine, and amino acid neurotransmitters revealed significant reductions in the levels of dopamine (p=0.004), norepinephrine (p=0.0009), epinephrine (p=0.0002), serotonin (p=0.004), and GABA (p=0.002) in the ALC group compared to the PF group. However, there was no significant change in the levels of glutamate in E13 fetal brains. These findings demonstrate that prenatal alcohol exposure reduces the concentrations of some catecholamines, idolamine, and amino acid neurotransmitters in E13 fetal brains. This study suggests that alterations of selective neurotransmitters may be the cause of abnormalities in brain function and behavior found in fetal alcohol spectrum disorders.
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影响因子：
2.9
作者：
BARRON, S;GAGNON, WA;RILEY, EP
通讯作者：
RILEY, EP
DOI：
10.1111/j.1749-6632.1990.tb16891.x
发表时间：
1990-10-15
影响因子：
5.2
作者：
LAUDER, JM
通讯作者：
LAUDER, JM
DOI：
10.1111/j.1530-0277.1995.tb01624.x
发表时间：
1995-10-01
影响因子：
3.2
作者：
MIDDAUGH, LD;BOGGAN, WO
通讯作者：
BOGGAN, WO
DOI：
10.1002/bdra.20440
发表时间：
2008-04-01
影响因子：
--
作者：
Datta, Susmita;Turner, Delano;Knudsen, Thomas B.
通讯作者：
Knudsen, Thomas B.
影响因子：
2.9
作者：
KORNGUTH, SE;RUTLEDGE, JJ;YOUNG, B
通讯作者：
YOUNG, B