Effect of a contact lens on mouse retinal in vivo imaging: Effective focal length changes and monochromatic aberrations.
Effect of a contact lens on mouse retinal in vivo imaging: Effective focal length changes and monochromatic aberrations.
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DOI：
10.1016/j.exer.2018.03.027
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发表时间：
2018-07
影响因子：
3.4
通讯作者：
Zawadzki RJ
中科院分区：
文献类型：
--
作者：
Zhang P;Mocci J;Wahl DJ;Meleppat RK;Manna SK;Quintavalla M;Muradore R;Sarunic MV;Bonora S;Pugh EN Jr;Zawadzki RJ
对于活体小鼠视网膜成像，特别是使用自适应光学仪器，使用隐形眼镜是可取的，因为它可以维持角膜水合，并有助于防止在长时间成像过程中形成白内障。然而，由于眼睛的屈光因素(角膜和晶状体)作为大多数活体视网膜成像系统的物镜，即使在0Dpt的情况下，也需要使用隐形眼镜。折射率，可以改变系统的光学性质。在这项调查中，我们检查了有效焦距的变化和使用隐形眼镜引起的像差。首先，用Zemax小鼠眼睛模型模拟焦距变化。然后在有和没有0度差的情况下进行眼睛像差。在定制的AO-SLO系统中，使用Shack-Hartmann波前传感器(SHWS)测量隐形眼镜。测量了两组小鼠(14月龄和2.5月龄)的总RMS波前误差，将其分解为66个Zernike像差项，并进行了比较。这些数据表明，在我们的系统中使用隐形眼镜会增加垂直彗差和球差。基于眼部波前数据，我们评估了隐形眼镜对成像系统性能的影响，作为瞳孔大小的函数。对两组小鼠的均方根误差和斯特雷尔比进行了量化，无论是否戴隐形眼镜，以及不同的输入光束大小。这些结果为在没有自适应光学的情况下确定视网膜成像的最佳瞳孔大小提供了信息，并为包含隐形眼镜的小鼠光学成像系统的设计提出了关键问题。
For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance of cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements of the eye (cornea and lens) serve as the objective for most in vivo retinal imaging systems, the use of a contact lens, even with 0 Dpt. refractive power, can alter the system’s optical properties. In this investigation we examined the effective focal length change and the aberrations that arise from use of a contact lens. First, focal length changes were simulated with a Zemax mouse eye model. Then ocular aberrations with and without a 0 Dpt. contact lens were measured with a Shack-Hartmann wavefront sensor (SHWS) in a customized AO-SLO system. Total RMS wavefront errors were measured for two groups of mice (14-month, and 2.5-month-old), decomposed into 66 Zernike aberration terms, and compared. These data revealed that vertical coma and spherical aberrations were increased with use of a contact lens in our system. Based on the ocular wavefront data we evaluated the effect of the contact lens on the imaging system performance as a function of the pupil size. Both RMS error and Strehl ratios were quantified for the two groups of mice, with and without contact lenses, and for different input beam sizes. These results provide information for determining optimum pupil size for retinal imaging without adaptive optics, and raise critical issues for design of mouse optical imaging systems that incorporate contact lenses.
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DOI：
10.1364/josaa.18.001793
发表时间：
2001-08-01
影响因子：
1.9
作者：
Porter, J;Guirao, A;Williams, DR
通讯作者：
Williams, DR
影响因子：
1.8
作者：
Paques, M;Sirnonutti, M;Sahel, JA
通讯作者：
Sahel, JA
影响因子：
2
作者：
Bermudez, Maria A.;Vicente, Ana F.;Gonzalez, Francisco
通讯作者：
Gonzalez, Francisco
影响因子：
3.6
作者：
Biss, David P.;Sumorok, Daniel;Lin, Charles P.
通讯作者：
Lin, Charles P.
影响因子：
3.4
作者：
Geng Y;Schery LA;Sharma R;Dubra A;Ahmad K;Libby RT;Williams DR
通讯作者：
Williams DR