Betabox: a beta particle imaging system based on a position sensitive avalanche photodiode.
Betabox: a beta particle imaging system based on a position sensitive avalanche photodiode.
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DOI：
10.1088/0031-9155/58/11/3739
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发表时间：
2013-06-07
影响因子：
3.5
通讯作者：
Chatziioannou AF
中科院分区：
文献类型：
--
作者：
Dooraghi AA;Vu NT;Silverman RW;Farrell R;Shah KS;Wang J;Heath JR;Chatziioannou AF
研制了一种β照相机，它可以利用14 × 14 mm 2的位置敏感雪崩光电二极管（PSAPD）对β粒子的空间和时间分布进行平面成像。这个摄像系统，我们称之为Betabox，可以直接连接到为细胞培养或其他生物应用设计的微流体芯片上。Betabox允许对这些芯片内标记有带电粒子发射体（如18F）的分子成像探针的细胞摄取进行成像。在这项工作中，我们研究了Betabox对18F β粒子的定量成像能力，包括本底率、效率、空间分辨率和计数率。背景和空间分辨率的测量在室温（21 °C ± 1 °C）和升高的操作温度（37 °C ± 1 °C）下都被考虑，如生物测定通常所要求的。在21 ° C和37 °C下，用4 keV能量截止测量的背景速率低于2 cph mm−2。Betabox用于检测与PSAPD接触的18F正电子源的绝对效率为46% ± 1%，PSAPD的表面钝化，不受环境光和损坏的影响。使用Rose准则估计的检测下限为0.2 cps mm−2（1 min采集）和62 × 62 µm2像素大小。检测上限约为21 000 cps。在21 ° C和37 °C下的空间分辨率范围从视场（FOV）中心处的0.4 mm FWHM开始，并且在距离中心5 mm处降低到1 mm，从而产生约10 × 10 mm2的有用FOV。我们还研究了使用基于聚合物的微流控芯片对空间分辨率和灵敏度的影响。在这些研究中，我们在探测器和光源之间放置了不同的低密度聚乙烯（LDPE）层，发现每100 µm的LDPE薄膜，空间分辨率会降低约180 µm。加入约200 µm的额外LDPE薄膜后，灵敏度降低一半。最后，我们展示了Betabox的实际利用，其线性的成像测试，当耦合到一个聚二甲基硅氧烷微流控芯片设计用于基于细胞的测定。
A beta camera has been developed that allows planar imaging of the spatial and temporal distribution of beta particles using a 14 × 14 mm2 position sensitive avalanche photodiode (PSAPD). This camera system, which we call Betabox, can be directly coupled to microfluidic chips designed for cell incubation or other biological applications. Betabox allows for imaging the cellular uptake of molecular imaging probes labeled with charged particle emitters such as 18F inside these chips. In this work, we investigate the quantitative imaging capabilities of Betabox for 18F beta particles, in terms of background rate, efficiency, spatial resolution, and count rate. Measurements of background and spatial resolution are considered both at room temperature (21 °C ± 1 °C) and at an elevated operating temperature (37 °C ± 1 °C), as is often required for biological assays. The background rate measured with a 4 keV energy cutoff is below 2 cph mm−2 at both 21 and 37 °C. The absolute efficiency of Betabox for the detection of 18F positron sources in contact with a PSAPD with the surface passivated from ambient light and damage is 46% ± 1%. The lower detection limit is estimated using the Rose Criterion to be 0.2 cps mm−2 for 1 min acquisitions and a 62 × 62 µm2 pixel size. The upper detection limit is approximately 21 000 cps. The spatial resolution at both 21 and 37 °C ranges from 0.4 mm FWHM at the center of the field of view (FOV), and degrades to 1 mm at a distance of 5 mm away from center yielding a useful FOV of approximately 10 × 10 mm2. We also investigate the effects on spatial resolution and sensitivity that result from the use of a polymer based microfluidic chip. For these studies we place varying layers of low-density polyethylene (LDPE) between the detector and the source and find that the spatial resolution degrades by ~180 µm for every 100 µm of LDPE film. Sensitivity is reduced by half with the inclusion of ~200 µm of additional LDPE film. Lastly, we demonstrate the practical utilization of Betabox, with an imaging test of its linearity, when coupled to a polydimethylsiloxane microfluidic chip designed for cell based assays.
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基于小细胞群体糖酵解的实时成像，与微流体芯片集成的Beta相机。
DOI：
10.2967/jnumed.110.078725
发表时间：
2011-05
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Vu NT;Yu ZT;Comin-Anduix B;Søndergaard JN;Silverman RW;Chang CY;Ribas A;Tseng HR;Chatziioannou AF
通讯作者：
Chatziioannou AF
影响因子：
3.5
作者：
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通讯作者：
Wells, K
DOI：
10.1016/s0168-9002(01)01724-7
发表时间：
2002-02-01
影响因子：
1.4
作者：
Bertolucci, E;Conti, M;Russo, P
通讯作者：
Russo, P
影响因子：
3.5
作者：
Russo, P.;Lauria, A.;Lastoria, S.
通讯作者：
Lastoria, S.
影响因子：
3.5
作者：
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通讯作者：
Wells, Kevin