Dissociation of circadian and circatidal timekeeping in the marine crustacean Eurydice pulchra.
Dissociation of circadian and circatidal timekeeping in the marine crustacean Eurydice pulchra.
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DOI：
10.1016/j.cub.2013.08.038
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发表时间：
2013-10-07
期刊：
影响因子：
9.2
通讯作者：
Wilcockson, David C.
中科院分区：
文献类型：
--
作者：
Zhang, Lin;Hastings, Michael H.;Green, Edward W.;Tauber, Eran;Sladek, Martin;Webster, Simon G.;Kyriacou, Charalambos P.;Wilcockson, David C.
潮汐(12.4小时)的行为和生理周期使潮间带生物适应暂时复杂的沿海环境，但其潜在机制尚不清楚。然而，独立的“昼夜节律”时钟的存在一直存在争议，而且有人争论说，潮汐节律作为昼夜节律时钟的亚倍数出现，在双振荡器中运行，其输出保持在反相，即∼12.4小时间隔。我们证明潮间带甲壳动物Eurydice Pulchra(LEACH)在行为、生理和分子表型上表现出与昼夜节律(24小时)平行的强劲的潮汐周期。重要的是，游泳的∼12.4小时循环是在恒定的条件下持续的，它们可以被适当的刺激携带，并且它们得到了温度补偿，从而满足了定义生物时钟的三个标准。出乎意料的是，潮汐节律(如昼夜节律)对酪蛋白激酶1的药物抑制很敏感，这表明共享时钟底物的可能性。然而，为了提供昼夜节律的分子标记以及通过RNAi扰乱昼夜节律的试剂，克隆了白头翁典型的昼夜节律基因，揭示了混淆昼夜节律的环境和分子操作不影响潮汐节律。因此，称职的昼夜节律计时既不是潮汐计时的必然要素，也不是必要要素。我们证明潮汐节律是由一个专门的昼夜节律起搏器驱动的，这种起搏器不同于普氏棘豆的昼夜节律系统，从而解决了关于昼夜节律机制的性质的长期争论。潮间带甲壳动物Eurydice Pulchra具有昼夜节律和潮汐表型我们已经克隆并表征了Eurydice的典型昼夜因子虽然对CK1抑制很敏感，但昼夜和潮汐时钟可以分离Eurydice有一个独立于其生物钟的专用昼夜时钟
Tidal (12.4 hr) cycles of behavior and physiology adapt intertidal organisms to temporally complex coastal environments, yet their underlying mechanism is unknown. However, the very existence of an independent “circatidal” clock has been disputed, and it has been argued that tidal rhythms arise as a submultiple of a circadian clock, operating in dual oscillators whose outputs are held in antiphase i.e., ∼12.4 hr apart. We demonstrate that the intertidal crustacean Eurydice pulchra (Leach) exhibits robust tidal cycles of swimming in parallel to circadian (24 hr) rhythms in behavioral, physiological and molecular phenotypes. Importantly, ∼12.4 hr cycles of swimming are sustained in constant conditions, they can be entrained by suitable stimuli, and they are temperature compensated, thereby meeting the three criteria that define a biological clock. Unexpectedly, tidal rhythms (like circadian rhythms) are sensitive to pharmacological inhibition of Casein kinase 1, suggesting the possibility of shared clock substrates. However, cloning the canonical circadian genes of E. pulchra to provide molecular markers of circadian timing and also reagents to disrupt it by RNAi revealed that environmental and molecular manipulations that confound circadian timing do not affect tidal timing. Thus, competent circadian timing is neither an inevitable nor necessary element of tidal timekeeping. We demonstrate that tidal rhythms are driven by a dedicated circatidal pacemaker that is distinct from the circadian system of E. pulchra, thereby resolving a long-standing debate regarding the nature of the circatidal mechanism. The intertidal crustacean Eurydice pulchra exhibits circadian and tidal phenotypes We have cloned and characterized the canonical circadian factors of Eurydice While sensitive to CK1 inhibition, circadian and tidal clocks can be dissociated Eurydice has a dedicated circatidal clock independent of its circadian clock
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