Adhesion and growth of dental pulp stem cells on enamel-like fluorapatite surfaces.
Adhesion and growth of dental pulp stem cells on enamel-like fluorapatite surfaces.
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DOI：
10.1002/jbm.a.33002
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发表时间：
2011-03-01
影响因子：
4.9
通讯作者：
Clarkson, B. H.
中科院分区：
文献类型：
--
作者：
Liu, J.;Jin, T. C.;Chang, S.;Czajka-Jakubowska, A.;Clarkson, B. H.
研究牙釉质样基质的磷灰石晶体取向对DPSC细胞黏附和生长的影响，为将来的研究提供体外牙釉质/牙本质超结构的前体。细胞在10%胎牛血清DMEM中进行传代培养，传代时间为7周。标本在扫描电子显微镜下观察、计数，并使用人类通路聚焦基质和黏附PCR阵列进行分析。3天后，FA有序表面的细胞数显著高于无序表面。在84个聚焦通路基因中，与无序表面相比，有20个基因在有序的FA表面表达上调或下调。更有趣的是，在细胞-基质黏附分子中，整合素α7和8(ITGA 7和8)、整合素β3和4(ITGB3和4)、玻璃体连接蛋白受体整合素αV(ITGAV)和关键黏附蛋白纤维连接蛋白1(FN1)表达上调。扫描电子显微镜下，两种表面均表现出良好的生物相容性，并支持DPSC细胞的长期生长，但在有序的FA表面上存在细胞-基质的功能性相互作用。DPSC细胞对有序FA晶体表面的增强细胞反应涉及一组微调的基质和黏附分子，可以通过用牙本质提取物处理细胞来操纵这些基质和黏附分子，以产生牙本质/釉质超结构。
To study how apatite crystal alignment of an enamel-like substrate affects DPSC cellular adhesion and growth as a precursor to produce an in vitro enamel/dentin superstructure for future studies. The cells were subcultured in 10% FBS DMEM up to 7 weeks on the two surfaces. Specimens were observed under SEM, counted, and analyzed using the human pathway-focused matrix and adhesion PCR array. After 3 days, the cell number on ordered FA surface was significantly higher than on the disordered surface. Of the 84 focused pathway genes, a total of 20 genes were either up or down regulated in the cells on ordered FA surface compared to the disordered surface. More interestingly, of the cell-matrix adhesion molecules, integrin alpha 7 and 8 (ITGA 7 and 8), integrin beta 3 and 4 (ITGB3 and 4), and the vitronectin receptor-integrin alpha V (ITGAV) and the key adhesion protein-fibronectin1 (FN1) were up-regulated. In SEM, both surfaces showed good biocompatibility and supported long term growth of DPSC cells but with functional cell-matrix interaction on the ordered FA surfaces. The enhanced cellular response of DPSC cell to the ordered FA crystal surface involves a set of delicately regulated matrix and adhesion molecules which could be manipulated by treating the cells with a dentin extract, to produce a dentin/enamel superstructure.
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影响因子：
3.1
作者：
Shi, S;Bartold, P M;Gronthos, S
通讯作者：
Gronthos, S
影响因子：
14
作者：
Curran, Judith M.;Chen, Rui;Hunt, John A.
通讯作者：
Hunt, John A.
影响因子：
3
作者：
LINDE, A;JOHANSSON, S;JONTELL, M
通讯作者：
JONTELL, M
影响因子：
4.1
作者：
Liu, Jun;Jin, Taocong;Clarkson, Brian H.
通讯作者：
Clarkson, Brian H.
影响因子：
14
作者：
STEELE, JG;DALTON, BA;UNDERWOOD, PA
通讯作者：
UNDERWOOD, PA