Chromosome painting and its applications in cultivated and wild rice.
Chromosome painting and its applications in cultivated and wild rice.
复制标题
染色体涂色及其在栽培稻和野生稻中的应用
DOI：
10.1186/s12870-018-1325-2
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发表时间：
2018-06-07
影响因子：
5.3
通讯作者：
Yu H
中科院分区：
文献类型：
--
作者：
Hou L;Xu M;Zhang T;Xu Z;Wang W;Zhang J;Yu M;Ji W;Zhu C;Gong Z;Gu M;Jiang J;Yu H
背景染色体特异性探针是染色体涂绘的基本工具，在哺乳动物中已得到广泛应用。然而，由于缺乏探针开发的方法，该技术尚未在植物中广泛应用。鉴定和标记大量的寡核苷酸（寡核苷酸）特定于一个单一的染色体为我们提供了一个机会，建立染色体特异性探针在植物。结果根据日本粳稻（Oryza sativaL.，AA，2n = 2× = 24）。用该探针可以很容易地识别出粳稻和籼稻的9号染色体。该探针还成功地用于鉴定和表征O. sativa、一个易位系、两个新的9号染色体非整倍体和一个野生稻（Oryza eichingeriA. Peter，CC，2n = 2× = 24）.结论本研究揭示了一个染色体特异性寡核苷酸池是一个有用的工具，用于水稻染色体彩绘。
BackgroundThe chromosome-specific probe is a fundamental tool of chromosome painting and has been commonly applied in mammalian species. The technology, however, has not been widely applied in plants due to a lack of methodologies for probe development. Identification and labeling of a large number of oligonucleotides (oligos) specific to a single chromosome offers us an opportunity to establish chromosome-specific probes in plants. However, never before has whole chromosome painting been performed in rice.ResultsWe developed a pooled chromosome 9-specific probe in rice, which contains 25,000 oligos based on the genome sequence of ajaponicarice (Oryza sativaL., AA, 2n = 2× = 24). Chromosome 9 was easily identified in bothjaponicaandindicarice using this chromosome 9-painting probe. The probe was also successfully used to identify and characterize chromosome 9 in additional lines ofO. sativa, a translocation line, two new aneuploids associated with chromosome 9 and a wild rice (Oryza eichingeriA. Peter, CC, 2n = 2× = 24).ConclusionThe study reveals that a pool of oligos specific to a chromosome is a useful tool for chromosome painting in rice.
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影响因子：
1.6
作者：
Idziak D;Betekhtin A;Wolny E;Lesniewska K;Wright J;Febrer M;Bevan MW;Jenkins G;Hasterok R
通讯作者：
Hasterok R
DOI：
10.1101/sqb.1993.058.01.085
发表时间：
1993-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
CREMER, T;KURZ, A;LICHTER, P
通讯作者：
LICHTER, P
DOI：
10.1007/bf00283259
发表时间：
1994-10-28
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
KAMISUGI, Y;NAKAYAMA, S;FUKUI, K
通讯作者：
FUKUI, K
影响因子：
5.4
作者：
Aggarwal, RK;Brar, DS;Khush, GS
通讯作者：
Khush, GS
影响因子：
1.3
作者：
Graphodatsky AS;Trifonov VA;Stanyon R
通讯作者：
Stanyon R