Bioaccumulation of Cry1Ab protein from an herbivore reduces anti-oxidant enzyme activities in two spider species.
Bioaccumulation of Cry1Ab protein from an herbivore reduces anti-oxidant enzyme activities in two spider species.
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来自草食动物的 Cry1Ab 蛋白的生物积累降低了两种蜘蛛的抗氧化酶活性
DOI：
10.1371/journal.pone.0084724
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Song Q
中科院分区：
文献类型：
--
作者：
Zhou J;Xiao K;Wei B;Wang Z;Tian Y;Tian Y;Song Q
Cry蛋白在水稻品系中表达用于鳞翅目害虫控制。这些蛋白质可以从转基因水稻植物转移到非目标节肢动物，包括稻飞虱，然后转移到捕食性蜘蛛。Cry蛋白通过食物网的移动可能会降低非目标节肢动物的适应性，尽管最近的出版物表明非目标种群没有发生严重变化。然而，Cry蛋白中毒影响Cry敏感昆虫中的基因表达。我们提出了Cry蛋白中毒影响蜘蛛在三营养食物网中作用的酶活性的假设。在这里，我们报告的结果，旨在测试我们的假设与两个蜘蛛物种的实验。我们证明了CryAb蛋白从果蝇培养基到果蝇的运动维持在含有CryAb的培养基上，并且从果蝇到蜘蛛Ummeliata insecticeps和Pardosa pseudoannulata。我们还表明，三个关键的代谢酶，乙酰胆碱酯酶（AchE），谷胱甘肽过氧化物酶（GSH-Px），和超氧化物歧化酶（SOD）的活性显着影响后，在蜘蛛喂食含Cry 1Ab的果蝇。我们从这些数据中推断，来自转基因作物的Cry蛋白在生理和生化水平上影响非靶标节肢动物，这可能是Cry蛋白相关的非靶标有益捕食者适合度降低的机制之一。
Cry proteins are expressed in rice lines for lepidopteran pest control. These proteins can be transferred from transgenic rice plants to non-target arthropods, including planthoppers and then to a predatory spider. Movement of Cry proteins through food webs may reduce fitness of non-target arthropods, although recent publications indicated no serious changes in non-target populations. Nonetheless, Cry protein intoxication influences gene expression in Cry-sensitive insects. We posed the hypothesis that Cry protein intoxication influences enzyme activities in spiders acting in tri-trophic food webs. Here we report on the outcomes of experiments designed to test our hypothesis with two spider species. We demonstrated that the movement of CryAb protein from Drosophila culture medium into fruit flies maintained on the CryAb containing medium and from the flies to the spiders Ummeliata insecticeps and Pardosa pseudoannulata. We also show that the activities of three key metabolic enzymes, acetylcholine esterase (AchE), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) were significantly influenced in the spiders after feeding on Cry1Ab-containing fruit flies. We infer from these data that Cry proteins originating in transgenic crops impacts non-target arthropods at the physiological and biochemical levels, which may be one mechanism of Cry protein-related reductions in fitness of non-target beneficial predators.
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影响因子：
2.2
作者：
Ye, GY;Shu, QY;Altosaar, I
通讯作者：
Altosaar, I
影响因子：
4.1
作者：
Ye, Rongjian;Huang, Haiqun;Lin, Yongjun
通讯作者：
Lin, Yongjun
影响因子：
4.2
作者：
Chen, Mao;Liu, Zhi-Cheng;Shelton, Anthony M.
通讯作者：
Shelton, Anthony M.
影响因子：
3.7
作者：
Tian JC;Chen Y;Li ZL;Li K;Chen M;Peng YF;Hu C;Shelton AM;Ye GY
通讯作者：
Ye GY
影响因子：
3.1
作者：
Riaz, N.;Husnain, T.;Riazuddin, S.
通讯作者：
Riazuddin, S.