The cannabinoid-1 receptor is abundantly expressed in striatal striosomes and striosome-dendron bouquets of the substantia nigra.
The cannabinoid-1 receptor is abundantly expressed in striatal striosomes and striosome-dendron bouquets of the substantia nigra.
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DOI：
10.1371/journal.pone.0191436
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Lovinger DM
中科院分区：
文献类型：
--
作者：
Davis MI;Crittenden JR;Feng AY;Kupferschmidt DA;Naydenov A;Stella N;Graybiel AM;Lovinger DM
突触前大麻素1受体(CB1-R)结合内源性和外源性大麻素，调节神经递质的释放。CB1受体表达于整个基底节，包括纹状体和黑质，它们在学习和控制动机行为方面发挥作用。然而，CB1-R在不同纹状体隔间、微回路和传出靶区的表达模式，以及不同表达CB1-R的神经元对这种模式的贡献尚不清楚。我们使用常规技术和新的遗传模型相结合的方法来评估CB1-R在纹状体的纹状体(斑块)和基质隔室中的表达，以及在纹状体中棘突投射神经元(MSN)的黑质靶中的表达。CB1-R蛋白和mRNA在尾侧纹状体遵循从背外侧到腹内侧的递减强度梯度，在纹状体中的表达高于周围基质。纹状体CB1-Rs的侧向优势与经典的纹状体标志物--mU阿片受体(MOR)的优势形成对比，后者最显著地表达于纹状体内侧。背外侧至腹内侧CB1-R的梯度类似于Drd2多巴胺受体的免疫反应，而与P物质相反。这种CB1-R的表达拓扑结构维持在苍白球和黑质的下游。密集的表达CB1-R的纹状体纤维在黑质网状部的背侧延伸，并与黑质致密部的腹侧延伸的纹状体靶向的多巴胺神经元树突(纹状体-树突束)共存。在纹状体内，CB1-Rs与荧光标记的MSN侧支共存于纹状体内。Cre重组酶介导的CB1-Rs从皮质投射神经元或MSN中的缺失，以及在基因敲除小鼠中MSN选择性地重新引入CB1-Rs，表明背外侧纹状体中CB1-Rs的主要来源是局部MSN侧支。这些数据表明，CB1受体在纹状体尾侧背外侧侧支和纹状体-树突束向黑质外侧的投射中起作用，在那里它们在解剖学上准备介导纹状体MSN和中脑多巴胺神经元对内源性大麻素和大麻仿药的突触前去抑制。
Presynaptic cannabinoid-1 receptors (CB1-R) bind endogenous and exogenous cannabinoids to modulate neurotransmitter release. CB1-Rs are expressed throughout the basal ganglia, including striatum and substantia nigra, where they play a role in learning and control of motivated actions. However, the pattern of CB1-R expression across different striatal compartments, microcircuits and efferent targets, and the contribution of different CB1-R-expressing neurons to this pattern, are unclear. We use a combination of conventional techniques and novel genetic models to evaluate CB1-R expression in striosome (patch) and matrix compartments of the striatum, and in nigral targets of striatal medium spiny projection neurons (MSNs). CB1-R protein and mRNA follow a descending dorsolateral-to-ventromedial intensity gradient in the caudal striatum, with elevated expression in striosomes relative to the surrounding matrix. The lateral predominance of striosome CB1-Rs contrasts with that of the classical striosomal marker, the mu opioid receptor (MOR), which is expressed most prominently in rostromedial striosomes. The dorsolateral-to-ventromedial CB1-R gradient is similar to Drd2 dopamine receptor immunoreactivity and opposite to Substance P. This topology of CB1-R expression is maintained downstream in the globus pallidus and substantia nigra. Dense CB1-R-expressing striatonigral fibers extend dorsally within the substantia nigra pars reticulata, and colocalize with bundles of ventrally extending, striosome-targeted, dendrites of dopamine-containing neurons in the substantia nigra pars compacta (striosome-dendron bouquets). Within striatum, CB1-Rs colocalize with fluorescently labeled MSN collaterals within the striosomes. Cre recombinase-mediated deletion of CB1-Rs from cortical projection neurons or MSNs, and MSN-selective reintroduction of CB1-Rs in knockout mice, demonstrate that the principal source of CB1-Rs in dorsolateral striosomes is local MSN collaterals. These data suggest a role for CB1-Rs in caudal dorsolateral striosome collaterals and striosome-dendron bouquet projections to lateral substantia nigra, where they are anatomically poised to mediate presynaptic disinhibition of both striosomal MSNs and midbrain dopamine neurons in response to endocannabinoids and cannabinomimetics.
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影响因子：
16.2
作者：
Banghart MR;Neufeld SQ;Wong NC;Sabatini BL
通讯作者：
Sabatini BL
DOI：
10.1073/pnas.1613337113
发表时间：
2016-10-04
影响因子：
11.1
作者：
Crittenden, Jill R.;Tillberg, Paul W.;Graybiel, Ann M.
通讯作者：
Graybiel, Ann M.
影响因子：
2.5
作者：
GERFEN, CR
通讯作者：
GERFEN, CR
影响因子：
4.7
作者：
Covey DP;Mateo Y;Sulzer D;Cheer JF;Lovinger DM
通讯作者：
Lovinger DM
伏隔核中GABA能中型棘神经元的分布和分室组织。
影响因子：
3.5
作者：
Gangarossa G;Espallergues J;de Kerchove d'Exaerde A;El Mestikawy S;Gerfen CR;Hervé D;Girault JA;Valjent E
通讯作者：
Valjent E