Wolbachia-mediated antibacterial protection and immune gene regulation in Drosophila.
Wolbachia-mediated antibacterial protection and immune gene regulation in Drosophila.
复制标题
果蝇中沃尔巴氏菌介导的抗菌保护和免疫基因调节。
DOI：
10.1371/journal.pone.0025430
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Johnson KN
中科院分区：
文献类型：
--
作者：
Wong ZS;Hedges LM;Brownlie JC;Johnson KN
微生物感染昆虫的结果不仅取决于宿主和病原体之间的相互作用，而且还取决于共感染宿主的微生物之间的相互作用。最近，母系遗传的内共生细菌沃尔巴克氏体已被证明可以保护昆虫免受一系列微生物和真核病原体的侵害。实验性感染沃尔巴克氏体的蚊子具有上调的免疫应答，并且免受许多病原体的侵害，包括病毒、细菌、疟原虫和丝虫线虫。据推测，免疫上调支持沃尔巴克氏体介导的保护。果蝇是理解宿主-沃尔巴克氏体-病原体相互作用的一个强有力的模型。在果蝇中，沃尔巴克氏体介导的抗病毒保护作用已被证明是针对许多不同的沃尔巴克氏体菌株。在这项研究中，我们调查了受沃尔巴克氏体感染的苍蝇是否也能免受病原菌的侵害。果蝇simulans线感染五种不同的沃尔巴克氏体菌株的挑战与病原菌铜绿假单胞菌PA 01，粘质沙雷氏菌和欧文氏菌carotovora和死亡率相比，配对线没有沃尔巴克氏体。在有或没有沃尔巴克氏体的果蝇中没有观察到死亡率的差异。同样，没有观察到D.感染了沃尔巴克氏体有趣的是，D.与不含沃尔巴克氏体的成对果蝇相比，自然感染沃尔巴克氏体的黑腹果蝇俄勒冈州RC果蝇的抗菌免疫基因TepIV、防御素、Diptericin B、PGRP-SD、抗菌肽A1和Attacin D没有上调。总之，这些结果表明，沃尔巴克氏体介导的抗菌保护是不普遍存在于昆虫，此外，抗菌和抗病毒保护的机制是独立的。我们认为，免疫启动和抗菌保护观察沃尔巴克氏体感染的蚊子可能是一个后果，最近人工引入的共生体到昆虫，通常不携带沃尔巴克氏体和抗菌保护是不太可能被发现在携带长期沃尔巴克氏体感染的昆虫。
The outcome of microbial infection of insects is dependent not only on interactions between the host and pathogen, but also on the interactions between microbes that co-infect the host. Recently the maternally inherited endosymbiotic bacteria Wolbachia has been shown to protect insects from a range of microbial and eukaryotic pathogens. Mosquitoes experimentally infected with Wolbachia have upregulated immune responses and are protected from a number of pathogens including viruses, bacteria, Plasmodium and filarial nematodes. It has been hypothesised that immune upregulation underpins Wolbachia-mediated protection. Drosophila is a strong model for understanding host-Wolbachia-pathogen interactions. Wolbachia-mediated antiviral protection in Drosophila has been demonstrated for a number of different Wolbachia strains. In this study we investigate whether Wolbachia-infected flies are also protected against pathogenic bacteria. Drosophila simulans lines infected with five different Wolbachia strains were challenged with the pathogenic bacteria Pseudomonas aeruginosa PA01, Serratia marcescens and Erwinia carotovora and mortality compared to paired lines without Wolbachia. No difference in mortality was observed in the flies with or without Wolbachia. Similarly no antibacterial protection was observed for D. melanogaster infected with Wolbachia. Interestingly, D. melanogaster Oregon RC flies which are naturally infected with Wolbachia showed no upregulation of the antibacterial immune genes TepIV, Defensin, Diptericin B, PGRP-SD, Cecropin A1 and Attacin D compared to paired flies without Wolbachia. Taken together these results indicate that Wolbachia-mediated antibacterial protection is not ubiquitous in insects and furthermore that the mechanisms of antibacterial and antiviral protection are independent. We suggest that the immune priming and antibacterial protection observed in Wolbachia-infected mosquitoes may be a consequence of the recent artificial introduction of the symbiont into insects that normally do not carry Wolbachia and that antibacterial protection is unlikely to be found in insects carrying long-term Wolbachia infections.
登录
查看更多内容
影响因子：
3.7
作者：
Chevalier F;Herbinière-Gaboreau J;Bertaux J;Raimond M;Morel F;Bouchon D;Grève P;Braquart-Varnier C
通讯作者：
Braquart-Varnier C
影响因子：
3.8
作者：
Hedges, Lauren M.;Johnson, Karyn N.
通讯作者：
Johnson, Karyn N.
影响因子：
6.7
作者：
Kambris Z;Blagborough AM;Pinto SB;Blagrove MS;Godfray HC;Sinden RE;Sinkins SP
通讯作者：
Sinkins SP
影响因子：
3.8
作者：
Osborne, RJ;Symonds, TM;Dall, DJ
通讯作者：
Dall, DJ
影响因子：
2.8
作者：
Casiraghi, M;Bordenstein, SR;Bandi, C
通讯作者：
Bandi, C