TMT-Based Quantitative Proteomic Analysis Reveals the Physiological Regulatory Networks of Embryo Dehydration Protection in Lotus (Nelumbo nucifera).
TMT-Based Quantitative Proteomic Analysis Reveals the Physiological Regulatory Networks of Embryo Dehydration Protection in Lotus (Nelumbo nucifera).
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DOI：
10.3389/fpls.2021.792057
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发表时间：
2021
影响因子：
5.6
通讯作者：
Ren L
中科院分区：
文献类型：
--
作者：
Zhang D;Liu T;Sheng J;Lv S;Ren L
荷花是水生植物，对水分流失敏感，但其种子在种胚脱水成熟后寿命较长。营养器官和种子对脱水反应的巨大差异与胚胎中特殊的保护机制有关。在这项研究中，利用串联质量标签（TMT）标记的蛋白质组学和平行反应监测（PRM）技术来获得对莲子脱水过程中生理调节网络的新见解。总共检测到 60,266 个二级光谱和 32,093 个独特肽。基于TMT数据共鉴定出5,477个蛋白和815个差异表达蛋白（DEP）。其中，582个DEP在整个脱水过程中持续下调，228个蛋白质显着上调。生物信息学和蛋白质-蛋白质相互作用网络分析表明，碳水化合物代谢（包括糖酵解/糖异生、半乳糖、淀粉和蔗糖代谢、磷酸戊糖途径和细胞壁组织）、内质网蛋白质加工、DNA修复和抗氧化事件对莲胚脱水有正向反应。相反，在莲胚失水和成熟过程中，能量代谢（代谢途径、光合作用、丙酮酸代谢、脂肪酸生物合成）和次生代谢（萜类骨架、类固醇、黄酮类生物合成）逐渐趋于静止状态。此外，非酶抗氧化剂和磷酸戊糖途径在莲胚脱水过程中的抗氧化保护中发挥着重要作用。脱落酸(ABA)信号传导以及寡糖、胚胎发生后期丰富蛋白和热休克蛋白的积累可能是保证莲子胚持续脱水和耐贮藏的关键因素。应激生理检测表明，H2O2是诱导氧化应激损伤的主要活性氧（ROS）成分，谷胱甘肽和维生素E是脱水过程中维持莲胚氧化还原平衡的主要抗氧化剂。这些结果为揭示莲花胚胎脱水保护机制的生理调控网络提供了新的见解。
Lotus is an aquatic plant that is sensitive to water loss, but its seeds are longevous after seed embryo dehydration and maturation. The great difference between the responses of vegetative organs and seeds to dehydration is related to the special protective mechanism in embryos. In this study, tandem mass tags (TMT)-labeled proteomics and parallel reaction monitoring (PRM) technologies were used to obtain novel insights into the physiological regulatory networks during lotus seed dehydration process. Totally, 60,266 secondary spectra and 32,093 unique peptides were detected. A total of 5,477 proteins and 815 differentially expressed proteins (DEPs) were identified based on TMT data. Of these, 582 DEPs were continuously downregulated and 228 proteins were significantly up-regulated during the whole dehydration process. Bioinformatics and protein-protein interaction network analyses indicated that carbohydrate metabolism (including glycolysis/gluconeogenesis, galactose, starch and sucrose metabolism, pentose phosphate pathway, and cell wall organization), protein processing in ER, DNA repair, and antioxidative events had positive responses to lotus embryo dehydration. On the contrary, energy metabolism (metabolic pathway, photosynthesis, pyruvate metabolism, fatty acid biosynthesis) and secondary metabolism (terpenoid backbone, steroid, flavonoid biosynthesis) gradually become static status during lotus embryo water loss and maturation. Furthermore, non-enzymatic antioxidants and pentose phosphate pathway play major roles in antioxidant protection during dehydration process in lotus embryo. Abscisic acid (ABA) signaling and the accumulation of oligosaccharides, late embryogenesis abundant proteins, and heat shock proteins may be the key factors to ensure the continuous dehydration and storage tolerance of lotus seed embryo. Stress physiology detection showed that H2O2 was the main reactive oxygen species (ROS) component inducing oxidative stress damage, and glutathione and vitamin E acted as the major antioxidant to maintain the REDOX balance of lotus embryo during the dehydration process. These results provide new insights to reveal the physiological regulatory networks of the protective mechanism of embryo dehydration in lotus.
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影响因子：
4.4
作者：
Gong M;Zhang H;Wu D;Zhang Z;Zhang J;Bao D;Yang Y
通讯作者：
Yang Y
类似稻草人的15与组蛋白脱乙酰基酶相互作用，对于抑制种子成熟程序至关重要。
影响因子：
16.6
作者：
Gao MJ;Li X;Huang J;Gropp GM;Gjetvaj B;Lindsay DL;Wei S;Coutu C;Chen Z;Wan XC;Hannoufa A;Lydiate DJ;Gruber MY;Chen ZJ;Hegedus DD
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4.3
作者：
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6.9
作者：
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影响因子：
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作者：
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通讯作者：
Heckathorn, SA