Aspirin promotes osteogenic differentiation of human dental pulp stem cells.
Aspirin promotes osteogenic differentiation of human dental pulp stem cells.
复制标题
阿司匹林促进人牙髓干细胞成骨分化
DOI：
10.3892/ijmm.2018.3801
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发表时间：
2018-10
影响因子：
5.4
通讯作者：
Zhang B
中科院分区：
文献类型：
--
作者：
Yuan M;Zhan Y;Hu W;Li Y;Xie X;Miao N;Jin H;Zhang B
人牙髓干细胞（hDPSC）具有自我更新和成骨分化特性，已用于口面部骨再生和牙周治疗。阿司匹林已被证明可以增强骨髓间充质干细胞（MSC）的再生；然而，阿司匹林对 hDPSCs 成骨分化的影响仍不清楚。在本研究中，通过流式细胞术对 hDPSC 进行了表征，并使用茜素红、油红 O 和阿新蓝染色评估了它们的克隆形成潜力和多能性。使用 Cell Counting Kit-8 测定评估阿司匹林对 hDPSC 活力的影响。通过碱性磷酸酶活性、茜素红染色、逆转录聚合酶链反应和蛋白质印迹检查成骨能力。此外，在 Sprague-Dawley 大鼠中建立了体内颅骨缺损，以评估阿司匹林对基于 hDPSC 的骨再生的影响。无机牛骨被用作骨替代材料和hDPSCs的载体。通过放射线照相和组织学分析观察新骨形成。研究表明hDPSCs表达MSC标志物并在体外具有多能性。浓度≤100 μg/ml的阿司匹林对hDPSCs无毒性，并在体外增强hDPSCs的成骨作用。在植入后8周或12周时，阿司匹林显着增加大鼠颅骨缺损模型中基于hDPSC的骨形成（P<0.05）。数据表明阿司匹林在体外和体内促进 hDPSC 的成骨潜力。总体而言，本研究表明阿司匹林可提高 hDPSC 的骨再生能力。
Human dental pulp stem cells (hDPSCs) possess self-renewal and osteogenic differentiation properties, and have been used for orofacial bone regeneration and periodontal treatment. Aspirin has been demonstrated to enhance the regeneration of bone marrow mesenchymal stem cells (MSCs); however, the impact of aspirin on the osteogenic differentiation of hDPSCs remains unknown. In the present study, hDPSCs were characterized by flow cytometry, while their clonogenic potential and multipotency were assessed using alizarin red, Oil red O and alcian blue staining. The effect of aspirin on hDPSC viability was assessed using Cell Counting Kit-8 assay. Osteogenic capacity was examined by alkaline phosphatase activity, alizarin red staining, reverse transcription-polymerase chain reaction and western blotting. Furthermore, in vivo cranial defects were established in Sprague-Dawley rats to evaluate the effect of aspirin on hDPSC-based bone regeneration. Anorganic bovine bone was used as a bone replacement material and as the carrier for hDPSCs. New bone formation was observed through radiographic and histological analysis. The study demonstrated that hDPSCs expressed MSC markers and possessed multipotency in vitro. Aspirin was non-toxic to hDPSCs at a concentration of ≤100 μg/ml and enhanced the osteogenesis of hDPSCs in vitro. Aspirin significantly increased hDPSC-based bone formation in the rat cranial defect model at 8 or 12 weeks post-implantation (P<0.05). The data suggested that aspirin promotes the osteogenic potential of hDPSCs in vitro and in vivo. Overall, the present study indicated that aspirin improves the bone regeneration capacity of hDPSCs.
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DOI：
10.1073/pnas.0508480102
发表时间：
2005-11-22
影响因子：
11.1
作者：
Sun, L;Blair, HC;Zaidi, M
通讯作者：
Zaidi, M
影响因子：
7.6
作者：
Liu, Y.;Chen, C.;Shi, S.
通讯作者：
Shi, S.
通过使用含有低人类血清百分比的培养基作为牛血清的临床级替代品，对人牙纸浆衍生的干细胞的分离和表征。
影响因子：
3.7
作者：
Ferro F;Spelat R;Beltrami AP;Cesselli D;Curcio F
通讯作者：
Curcio F
影响因子：
6.2
作者：
Shi, S;Gronthos, S
通讯作者：
Gronthos, S
通过不同浓度的β-甘油磷酸盐对牙髓干细胞分化的调节。
DOI：
10.3390/molecules17021219
发表时间：
2012-01-31
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Liu M;Sun Y;Liu Y;Yuan M;Zhang Z;Hu W
通讯作者：
Hu W