Recruitment, augmentation and apoptosis of rat osteoclasts in 1,25-(OH)2D3 response to short-term treatment with 1,25-dihydroxyvitamin D3 in vivo.
Recruitment, augmentation and apoptosis of rat osteoclasts in 1,25-(OH)2D3 response to short-term treatment with 1,25-dihydroxyvitamin D3 in vivo.
复制标题
DOI：
10.1186/1471-2474-3-16
复制
发表时间：
2002-06-07
影响因子：
2.3
通讯作者：
Scutt A
中科院分区：
文献类型：
--
作者：
Miao D;Scutt A
虽然对破骨细胞（OC）的形成和活性的调节了解很多，但对OC衰老知之甚少。特别是，在体内施用1，25-（OH）2D 3后观察到的OC的命运尚不清楚。有证据表明，OC的正常命运是经历细胞凋亡（程序性细胞死亡）。我们研究了短期应用大剂量1，25-（OH）2D 3对实验性大鼠OC凋亡的影响。OC募集，扩增和凋亡的可视化和定量染色抗酒石酸酸性磷酸酶（TRAP），双染色TRAP/ED 1或TRAP/DAPI，原位DNA片段末端标记和组织形态学分析。高剂量1，25-（OH）2D 3短期治疗增加了骨髓中OC前体的募集，导致OC数量的短暂增加。随后迅速增加凋亡OC的数量和随后的清除。OC对1，25-（OH）2D 3处理的反应是剂量和部位依赖性的;较高剂量产生更强、更快的反应，并且在胫骨中的反应始终比在椎骨中更强、更快。本研究表明：（1）募集后，OC通过凋亡从吸收部位移除;（2）TRAP和ED 1的联合使用可用于体内鉴定OC及其前体;（3）TRAP和DAPI的双重染色或原位DNA片段化末端标记可用于体内鉴定凋亡的OC。
Although much is known about the regulation of osteoclast (OC) formation and activity, little is known about OC senescence. In particular, the fate of of OC seen after 1,25-(OH)2D3 administration in vivo is unclear. There is evidence that the normal fate of OC is to undergo apoptosis (programmed cell death). We have investigated the effect of short-term application of high dose 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on OC apoptosis in an experimental rat model. OC recruitment, augmentation and apoptosis was visualised and quantitated by staining histochemically for tartrate resistant acid phosphatase (TRAP), double staining for TRAP/ED1 or TRAP/DAPI, in situ DNA fragmentation end labelling and histomorphometric analysis. Short-term treatment with high-dose 1,25-(OH)2D3 increased the recruitment of OC precursors in the bone marrow resulting in a short-lived increase in OC numbers. This was rapidly followed by an increase in the number of apoptotic OC and their subsequent removal. The response of OC to 1,25-(OH)2D3 treatment was dose and site dependent; higher doses producing stronger, more rapid responses and the response in the tibiae being consistently stronger and more rapid than in the vertebrae. This study demonstrates that (1) after recruitment, OC are removed from the resorption site by apoptosis (2) the combined use of TRAP and ED1 can be used to identify OC and their precursors in vivo (3) double staining for TRAP and DAPI or in situ DNA fragmentation end labelling can be used to identify apoptotic OC in vivo.
登录
查看更多内容
影响因子：
4.8
作者：
HATTERSLEY, G;KERBY, JA;CHAMBERS, TJ
通讯作者：
CHAMBERS, TJ
影响因子：
--
作者：
MARKS, SC;WALKER, DG
通讯作者：
WALKER, DG
DOI：
10.1006/bbrc.2001.6251
发表时间：
2002-01-18
影响因子：
3.1
作者：
Kitazawa, R;Kitazawa, S
通讯作者：
Kitazawa, S
影响因子：
4.1
作者：
BOLLERSLEV, J;GRAM, J;MOSEKILDE, L
通讯作者：
MOSEKILDE, L
DOI：
10.1007/bf00572903
发表时间：
1993-08-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
JAGER, A;RADLANSKI, RJ;GOTZ, W
通讯作者：
GOTZ, W