Dynamic topographical control of mesenchymal stem cells by culture on responsive poly(ε-caprolactone) surfaces.
Dynamic topographical control of mesenchymal stem cells by culture on responsive poly(ε-caprolactone) surfaces.
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DOI：
10.1002/adma.201100821
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发表时间：
2011-08-02
影响因子：
29.4
通讯作者：
Ashby, Valerie Sheares
中科院分区：
文献类型：
--
作者：
Le, Duy M.;Kulangara, Karina;Adler, Andrew F.;Leong, Kam W.;Ashby, Valerie Sheares
文献中有明确的新证据支持表面形貌对各种细胞表型的影响。[1-5]机械生物学的最新进展严重依赖于合成细胞外基质（ECM）模拟来研究细胞现象如何依赖于表面几何形状。同时，微/纳米制造技术的发展使得构建定义良好的表面阵列成为可能，这些阵列旨在模拟细胞外微环境许多不同大小和形状的图案，包括凹槽、柱状和凹状，已被用于研究各种细胞类型的体外反应，如成纤维细胞、成骨细胞、上皮细胞、神经细胞和最近的干细胞。[7-14]细胞地形相互作用在细胞生物学和生物医学工程中具有深远的意义。许多生物过程，如胚胎发生和血管生成，都受到这些相互作用的强烈影响。[4,15,16]此外，ECM感知异常与许多疾病状态有关，如心肌病、肌肉萎缩症和肿瘤发生。[17-19]目前，地形学也正在被探索作为一种机械地指导干细胞命运的手段，并将在下一代组织工程支架的设计中发挥重要作用。[13,20 - 23]然而，围绕细胞地形相互作用仍然存在重要的基本问题，创新的动态生物材料可能提供以前静态基质无法获得的新见解。因此，人们越来越努力地设计动态底物，使其能够在更仿生的环境中向细胞传递活跃的物理信号。[24-28]
There is clear, emerging evidence in the literature supporting the influence of surface topography on various cell phenotypes.[1–5] Recent advancements in mechanobiology have relied heavily on synthetic extracellular matrix (ECM) mimics to investigate how cellular phenomena are dependent upon surface geometry. Concurrent developments in micro/nanofabrication techniques have enabled the construction of well-defined surface arrays which aim to emulate the extracellular microenvironment.[6] Numerous patterns of different sizes and shapes including grooves, posts, and pits have been used to study the in vitro response of various cell types such as: fibroblasts, osteoblasts, epithelial cells, neuronal cells, and more recently stem cells.[7–14]Cell-topography interactions have far-reaching implications in cell biology and biomedical engineering. Many biological processes such as embryogenesis and angiogenesis are strongly influenced by these interactions.[4, 15, 16] Additionally, abnormalities in ECM sensing have been linked to many disease states such as cardiomyopathy, muscular dystrophy, and oncogenesis.[17–19] Topography is also currently being explored as a means to mechanically direct stem cell fate and will be important in the design of next generation tissue engineering scaffolds.[13, 20–23] However, there remain significant fundamental questions surrounding cell-topography interactions for which innovative, dynamic biomaterials may offer new insights not previously accessible by static substrates. Accordingly, there has been an increased effort to design dynamic substrates that can communicate active physical cues to cells in a more biomimetic context.[24–28]
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影响因子：
14
作者：
Johansson, F;Carlberg, P;Kanje, M
通讯作者：
Kanje, M
影响因子：
13.3
作者：
Martins, Albino;Pinho, Elisabete D.;Neves, Nuno M.
通讯作者：
Neves, Nuno M.
影响因子：
6.2
作者：
Finne, A;Albertsson, AC
通讯作者：
Albertsson, AC
影响因子：
14
作者：
Lam, Mai T.;Clem, William C.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
3.4
作者：
Kulangara, Karina;Leong, Kam W.
通讯作者：
Leong, Kam W.