Testing odor response stereotypy in the Drosophila mushroom body.
Testing odor response stereotypy in the Drosophila mushroom body.
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DOI：
10.1016/j.neuron.2008.07.040
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发表时间：
2008-09-25
期刊：
影响因子：
16.2
通讯作者：
Laurent G
中科院分区：
文献类型：
--
作者：
Murthy M;Fiete I;Laurent G
蘑菇体是苍蝇学习嗅觉所必需的。其主要神经元是凯尼恩细胞(KCs)，形成一个庞大的细胞群，通常根据解剖学标准分为三类(α/β，α‘/β’和γ)。它们的嗅觉输入来源的两个神经元群体，投射神经元(PNS)和PNS的突触前伙伴，嗅觉感觉神经元，可以通过它们的肾小球投射、特定基因的表达和它们的气味反应来单独识别。我们在这里问，KC是否同样可以单独识别。我们聚焦于~23个基因标记的α/βKC的子集，并在体内用全细胞膜片钳记录它们对气味的反应。这一样本的反应曲线没有提供动物之间重复的证据。此外，在基因标记的子集中，跨动物的反应与在更大样本中的所有KC中的反应一样多样化。相比之下，记录的PN气味反应按肾小球类型聚集。使用这些PN反应作为一个简单的KC模型的输入，我们发现测量的飞行间PN反应的变异性结合刻板的PN到KC的连接不能解释KC气味反应刻板印象的缺失。这些结果表明，不同个体果蝇之间的PN-KC连接存在差异。
The mushroom body is required for olfactory learning in flies. Its principal neurons, the Kenyon cells (KCs), form a large cell population, usually divided into three classes(α/β, α’/β’, and γ) based on anatomical criteria. Both neuronal populations from which their olfactory input derives, the projection neurons (PNs) and the presynaptic partners of the PNs, the olfactory sensory neurons, can be identified individually by their glomerular projections, the expression of specific genes, and their odor responses. We ask here whether KCs are similarly identifiable individually. We focused on a subset of ~23 genetically labeled α/β KCs, and recorded their responses to odors using whole-cell patch-clamp in vivo. Response profiles across this sample provided no evidence for repeats across animals. Further, across-animal responses were as diverse within the genetically-labeled subset as across all KCs in a larger sample. By contrast, recorded PN odor responses clustered by glomerular type. Using these PN responses as inputs to a simple KC model, we find that measured inter-fly PN response variability combined with stereotyped PN-to-KC connectivity cannot explain the absence of KC odor response stereotypy. These results suggest differences in PN-to-KC connectivity across individual flies.
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