Laminin nanofiber meshes that mimic morphological properties and bioactivity of basement membranes.
Laminin nanofiber meshes that mimic morphological properties and bioactivity of basement membranes.
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DOI：
10.1089/ten.tec.2007.0366
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Botchwey EA
中科院分区：
文献类型：
--
作者：
Neal RA;McClugage SG;Link MC;Sefcik LS;Ogle RC;Botchwey EA
基底膜蛋白层粘连蛋白 I 已广泛用作平面二维膜或三维形式的重构基底膜凝胶（例如基质胶），以支持体外细胞附着、生长和分化。在体内基底膜中，层粘连蛋白表现出纤维状形态，突出表明静电纺丝过程是体外重建此类纤维基质的理想方法。采用静电纺丝技术制造鼠层粘连蛋白 I 纳米纤维 (LNF) 网，其纤维尺寸、几何形状和真实基底膜的孔隙率。纯化的层粘连蛋白 I 被溶解并静电纺丝，用于纤维直径与聚合物溶液浓度、收集距离和流速的函数关系的参数研究。所得纤维直径范围为 90 至 300 nm，网状形态包含珠子。与之前描述的由胶原蛋白等蛋白质合成的纳米纤维 (NF) 不同，LNF 的网状结构在润湿时保留其结构特征，并且不需要通过化学交联进行固定，而化学交联通常会破坏细胞附着和其他生物活性。 LNF 网在没有化学交联的情况下在培养中至少 2 天保持其几何形状。 PC12 细胞在 LNF 基质上无需神经生长因子刺激即可延伸神经突。此外，与层粘连蛋白薄膜相比，LNF 显着提高了人类脂肪干细胞 (ASC) 的附着率和数量。 ASCs 是可行的，并在无血清培养基中保持与 LNF 网的附着至少 3 天，并在无血清培养基条件下 24 小时后延长神经突样过程，无需培养基添加剂诱导分化。 LNF 网片是一种用于体外细胞研究的新型基质，其特性可能是在体内组织工程应用中输送细胞的优异支架材料。
The basement membrane protein, laminin I, has been used broadly as a planar two-dimensional film or in a three-dimensional form as a reconstituted basement membrane gel such as Matrigel to support cellular attachment, growth, and differentiation in vitro. In basement membranes in vivo, laminin exhibits a fibrillar morphology, highlighting the electrospinning process as an ideal method to recreate such fibrous substrates in vitro. Electrospinning was employed to fabricate meshes of murine laminin I nanofibers (LNFs) with fiber size, geometry, and porosity of authentic basement membranes. Purified laminin I was solubilized and electrospun in parametric studies of fiber diameters as a function of polymer solution concentration, collecting distance, and flow rate. Resulting fiber diameters ranged from 90 to 300 nm with mesh morphologies containing beads. Unlike previously described nanofibers (NFs) synthesized from proteins such as collagen, meshes of LNFs retain their structural features when wetted and do not require fixation by chemical crosslinking, which often destroys cell attachment and other biological activity. The LNF meshes maintained their geometry for at least 2 days in culture without chemical crosslinking. PC12 cells extended neurites without nerve growth factor stimulation on LNF substrates. Additionally, LNFs significantly enhance both the rate and quantity of attachment of human adipose stem cells (ASCs) compared to laminin films. ASCs were viable and maintained attachment to LNF meshes in serum-free media for at least 3 days in culture and extended neurite-like processes after 24 h in serum-free media conditions without media additives to induce differentiation. LNF meshes are a novel substrate for cell studies in vitro, whose properties may be an excellent scaffold material for delivering cells in tissue engineering applications in vivo.
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影响因子：
14
作者：
Bashur, Chris A.;Dahlgren, Linda A.;Goldstein, Aaron S.
通讯作者：
Goldstein, Aaron S.
影响因子：
2.7
作者：
ROGERS, SL;LETOURNEAU, PC;FURCHT, LT
通讯作者：
FURCHT, LT
影响因子：
15
作者：
Kakade, Meghana V.;Givens, Steven;Rabolt, John F.
通讯作者：
Rabolt, John F.
影响因子：
14
作者：
Sheu, MT;Huang, JC;Ho, HO
通讯作者：
Ho, HO
DOI：
10.1002/jbm.b.30041
发表时间：
2004-08-15
影响因子：
3.4
作者：
Katti, DS;Robinson, KW;Laurencin, CT
通讯作者：
Laurencin, CT