In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices.
In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices.
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DOI：
10.1083/jcb.97.5.1648
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发表时间：
1983-11
期刊：
影响因子：
--
通讯作者：
Vassalli P
中科院分区：
文献类型：
--
作者：
Montesano R;Orci L;Vassalli P
我们研究了生长在三维胶原基质中的克隆毛细血管内皮细胞的行为。建立在胶原凝胶表面的细胞单层被第二层胶原蛋白覆盖。这导致内皮细胞的单层重新组织成一个由分支和吻合的毛细血管样管组成的网络。如电子显微镜所见，管子由至少两个细胞(横切面)组成，界定了狭窄的管腔。此外，在内皮细胞的腔面和胶原基质之间存在明显的基板材料。这些结果表明，毛细血管内皮细胞在体外具有形成具有良好定向细胞极性的血管样结构的能力。他们还表明，内皮细胞与胶原基质之间的适当拓扑关系，类似于体内发生的那种关系，对这一潜力的表达具有诱导作用。该培养体系为研究新血管形成(血管生成)的相关因素提供了一个简单的体外模型。
We have studied the behavior of cloned capillary endothelial cells grown inside a three dimensional collagen matrix. Cell monolayers established on the surface of collagen gels were covered with a second layer of collagen. This induced the monolayers of endothelial cells to reorganize into a network of branching and anastomosing capillary-like tubes. As seen by electron microscopy, the tubes were formed by at least two cells (in transverse sections) delimiting a narrow lumen. In addition, distinct basal lamina material was present between the abluminal face of the endothelial cells and the collagen matrix. These results showed that capillary endothelial cells have the capacity to form vessel-like structures with well-oriented cell polarity in vitro. They also suggest that an appropriate topological relationship of endothelial cells with collagen matrices, similar to that occurring in vivo, has an inducive role on the expression of this potential. This culture system provides a simple in vitro model for studying the factors involved in the formation of new blood vessels (angiogenesis).
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悬浮在胶原蛋白凝胶中的上皮可以失去极性，并表达迁移间充质细胞的特征。
影响因子：
7.8
作者：
Greenburg, G;Hay, E D
通讯作者：
Hay, E D
DOI：
10.1083/jcb.92.3.747
发表时间：
1982-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Haston WS;Shields JM;Wilkinson PC
通讯作者：
Wilkinson PC
影响因子：
7.8
作者：
HAEUPTLE, MT;SUARD, YLM;KRAEHENBUHL, JP
通讯作者：
KRAEHENBUHL, JP
影响因子：
2.7
作者：
BERNANKE, DH;MARKWALD, RR
通讯作者：
MARKWALD, RR
DOI：
10.1073/pnas.76.10.5217
发表时间：
1979-01-01
影响因子：
11.1
作者：
FOLKMAN, J;HAUDENSCHILD, CC;ZETTER, BR
通讯作者：
ZETTER, BR