Outer Membrane Porin F in E. coli Is Critical for Effective Predation by Bdellovibrio.
Outer Membrane Porin F in E. coli Is Critical for Effective Predation by Bdellovibrio.
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DOI：
10.1128/spectrum.03094-22
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发表时间：
2022-12-21
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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蛭弧菌和类似生物（BALO）是一种独特的细菌群，它们通过捕食其他细菌而生存，在后代出来完成生命周期之前从内部消耗它们来生长和复制。然而，这些捕食者识别猎物并将其与非猎物细菌区分开来的机制仍然不清楚。通过对不同大肠杆菌菌株的基因敲除和互补研究，我们发现噬菌蛭弧菌109 J菌株识别大肠杆菌外膜孔蛋白F（OmpF）。coli表面的活性，并对E.大肠杆菌EnvZ-OmpR调控系统显著影响捕食动力学。然而，OmpF并不是BALO识别猎物为B的唯一信号。bacteriovorus可能最终先于E. coli ΔompF突变体。此外，识别OmpF作为猎物表面结构依赖于猎物菌株，因为敲除其他猎物物种中的OmpF蛋白同源物，包括大肠杆菌、肺炎克雷伯氏菌和肠道沙门氏菌，并不总是降低捕食率。因此，虽然OmpF被发现是蛭弧菌有效识别和攻击E.大肠杆菌，未来的工作是需要确定其他猎物表面结构，这些捕食者承认。重要性噬菌蛭弧菌和类似生物（BALO）是革兰氏阴性捕食性细菌，它们通过穿透其他革兰氏阴性细菌的周质并从内部消耗它们来攻击其他革兰氏阴性细菌，以获得捕食者生长和复制所需的营养。然而，这些掠食者如何识别猎物仍然是一个谜。在这里，我们表明，外膜孔蛋白F（OmpF）在E。大肠杆菌能被B识别。噬菌株109 J，这种蛋白质的损失导致严重延迟捕食。然而，其他几个猎物物种的捕食是不依赖于这种蛋白质或其同系物的识别，这表明有其他结构的捕食者识别的猎物表面，尚未被发现。
Bdellovibrio and like organisms (BALOs) are a unique bacterial group that live by predating on other bacteria, consuming them from within to grow and replicate before the progeny come out to complete the life cycle. The mechanisms by which these predators recognize their prey and differentiate them from nonprey bacteria, however, are still not clear. Through genetic knockout and complementation studies in different Escherichia coli strains, we found that Bdellovibrio bacteriovorus strain 109J recognizes outer membrane porin F (OmpF) on the E. coli surface and that the activity of the E. coli EnvZ-OmpR regulatory system significantly impacts predation kinetics. OmpF is not the only signal by which BALOs recognize their prey, however, as B. bacteriovorus could eventually predate on the E. coli ΔompF mutant after prolonged incubation. Furthermore, recognizing OmpF as a prey surface structure was dependent on the prey strain, as knocking out OmpF protein homologues in other prey species, including Escherichia fergusonii, Klebsiella pneumoniae, and Salmonella enterica, did not always reduce the predation rate. Consequently, although OmpF was found to be an important surface component used by Bdellovibrio to efficiently recognize and attack E. coli, future work is needed to determine what other prey surface structures are recognized by these predators. IMPORTANCE Bdellovibrio bacteriovorus and like organisms (BALOs) are Gram-negative predatory bacteria that attack other Gram-negative bacteria by penetrating their periplasm and consuming them from within to obtain the nutrients necessary for the predator’s growth and replication. How these predators recognize their prey, however, has remained a mystery. Here, we show that the outer membrane porin F (OmpF) in E. coli is recognized by B. bacteriovorus strain 109J and that the loss of this protein leads to severely delayed predation. However, predation of several other prey species was not dependent on the recognition of this protein or its homologues, indicating that there are other structures recognized by the predators on the prey surface that are yet to be discovered.
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影响因子：
16.6
作者：
Chakraborty S;Winardhi RS;Morgan LK;Yan J;Kenney LJ
通讯作者：
Kenney LJ
影响因子：
9.8
作者：
Maffei E;Shaidullina A;Burkolter M;Heyer Y;Estermann F;Druelle V;Sauer P;Willi L;Michaelis S;Hilbi H;Thaler DS;Harms A
通讯作者：
Harms A
影响因子：
3.2
作者：
FORST, S;DELGADO, J;INOUYE, M
通讯作者：
INOUYE, M
影响因子：
11
作者：
Im, Hansol;Dwidar, Mohammed;Mitchell, Robert J.
通讯作者：
Mitchell, Robert J.
影响因子：
2.7
作者：
MIZUNO, T;MIZUSHIMA, S
通讯作者：
MIZUSHIMA, S