Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and Red Skeletal Muscle in a Rat Model of High-Fat Diet-Induced Obesity.
Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and Red Skeletal Muscle in a Rat Model of High-Fat Diet-Induced Obesity.
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在高脂肪饮食诱导的肥胖大鼠模型中，慢性大麻二酚给药对氧化应激和炎症反应的衰减与白色和红色骨骼肌中n-6/n-3 PUFA比率的改善有关。
DOI：
10.3390/nu13051603
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发表时间：
2021-05-11
期刊：
影响因子：
5.9
通讯作者：
Chabowski A
中科院分区：
文献类型：
--
作者：
Bielawiec P;Harasim-Symbor E;Sztolsztener K;Konstantynowicz-Nowicka K;Chabowski A
脂肪酸的消耗量急剧增加，超过了个人的营养需求，并导致许多代谢紊乱。最近的数据表明，越来越多的人对使用大麻二酚（CBD）作为治疗肥胖症的有益作用的药物感兴趣。因此，我们的目的是研究慢性CBD给药对白色和红色腓肠肌中不同脂质组分中n-6/n-3多不饱和脂肪酸（PUFA）比例、炎症途径和氧化应激参数的影响。所有设计的实验都是在Wistar大鼠上进行的，Wistar大鼠喂食高脂肪饮食（HFD）或标准啮齿动物饮食七周，随后注射CBD（10 mg/kg，每天一次，持续两周）或其载体。脂质含量和氧化应激参数进行了评估，分别使用气液色谱法（GLC），比色法和/或免疫酶法。通过蛋白质印迹法测量炎症途径的蛋白质的总表达。我们的研究结果表明，脂肪酸（FAs）供应过剩与增加的氧化应激和炎症反应，这导致在骨骼肌中的脂肪酸，特别是n-6 PUFAs的过度积累。我们发现CBD显著改善了n-6/n-3 PUFA比率，并将平衡转向抗炎性n-3 PUFA，特别是在红色腓肠肌中。此外，CBD防止了脂质过氧化产物的产生，并减弱了两种类型骨骼肌的炎症反应。总之，上述结果表明CBD在治疗肥胖和相关紊乱方面具有潜在的治疗特性。
The consumption of fatty acids has increased drastically, exceeding the nutritional requirements of an individual and leading to numerous metabolic disorders. Recent data indicate a growing interest in using cannabidiol (CBD) as an agent with beneficial effects in the treatment of obesity. Therefore, our aim was to investigate the influence of chronic CBD administration on the n-6/n-3 polyunsaturated fatty acids (PUFAs) ratio in different lipid fractions, inflammatory pathway and oxidative stress parameters in the white and red gastrocnemius muscle. All the designed experiments were performed on Wistar rats fed a high-fat diet (HFD) or a standard rodent diet for seven weeks and subsequently injected with CBD (10 mg/kg once daily for two weeks) or its vehicle. Lipid content and oxidative stress parameters were assessed using gas–liquid chromatography (GLC), colorimetric and/or immunoenzymatic methods, respectively. The total expression of proteins of an inflammatory pathway was measured by Western blotting. Our results revealed that fatty acids (FAs) oversupply is associated with an increasing oxidative stress and inflammatory response, which results in an excessive accumulation of FAs, especially of n-6 PUFAs, in skeletal muscles. We showed that CBD significantly improved the n-6/n-3 PUFA ratio and shifted the equilibrium towards anti-inflammatory n-3 PUFAs, particularly in the red gastrocnemius muscle. Additionally, CBD prevented generation of lipid peroxidation products and attenuated inflammatory response in both types of skeletal muscle. In summary, the results mentioned above indicate that CBD presents potential therapeutic properties with respect to the treatment of obesity and related disturbances.
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影响因子：
5.6
作者：
Lionetti L;Mollica MP;Sica R;Donizzetti I;Gifuni G;Pignalosa A;Cavaliere G;Putti R
通讯作者：
Putti R
DOI：
10.3390/antiox7070093
发表时间：
2018-07-18
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
Gallelli CA;Calcagnini S;Romano A;Koczwara JB;de Ceglia M;Dante D;Villani R;Giudetti AM;Cassano T;Gaetani S
通讯作者：
Gaetani S
影响因子：
3.7
作者：
Cavaliere G;Trinchese G;Bergamo P;De Filippo C;Mattace Raso G;Gifuni G;Putti R;Moni BH;Canani RB;Meli R;Mollica MP
通讯作者：
Mollica MP
影响因子：
--
作者：
Abdul-Ghani MA;DeFronzo RA
通讯作者：
DeFronzo RA
影响因子：
3.6
作者：
Cătoi AF;Pârvu A;Mureşan A;Busetto L
通讯作者：
Busetto L