mlstdbNet - distributed multi-locus sequence typing (MLST) databases.

mlstdbNet - distributed multi-locus sequence typing (MLST) databases.
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DOI:
10.1186/1471-2105-5-86
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发表时间:
2004-07-01
期刊:
影响因子:
3
通讯作者:
Maiden MC
Maiden MC
中科院分区:
生物学4区
文献类型:
--
作者:
Jolley KA;Chan MS;Maiden MC

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多位点序列分型(MLST)是一种分型方法,通过比较管家基因片段的序列,有助于区分微生物分离株。mlstdbNet软件能够实现分布式网络访问MLST数据库,这些数据库可以通过Internet广泛链接。该软件使多个分离株数据库能够查询包含等位基因谱和序列定义的单个谱数据库。这种分离使分离物数据库能够由各个实验室建立,每个实验室都根据特定项目的需要进行定制,并具有适当的访问限制,同时保持单一明确的概况和序列信息来源的优势。数据库是由Perl CGI脚本解析的XML文件描述的。该软件提供了大量的方法来查询数据库,并进一步分解和导出生成的结果。其他功能可以通过安装第三方(免费提供)工具来启用。为MLST数据库开发分布式结构提供了可伸缩性和灵活性,使参与中心能够保持对自己数据的所有权,而不会引起重复和数据完整性问题。
Multi-locus sequence typing (MLST) is a method of typing that facilitates the discrimination of microbial isolates by comparing the sequences of housekeeping gene fragments. The mlstdbNet software enables the implementation of distributed web-accessible MLST databases that can be linked widely over the Internet. The software enables multiple isolate databases to query a single profiles database that contains allelic profile and sequence definitions. This separation enables isolate databases to be established by individual laboratories, each customised to the needs of the particular project and with appropriate access restrictions, while maintaining the benefits of a single definitive source of profile and sequence information. Databases are described by an XML file that is parsed by a Perl CGI script. The software offers a large number of ways to query the databases and to further break down and export the results generated. Additional features can be enabled by installing third-party (freely available) tools. Development of a distributed structure for MLST databases offers scalability and flexibility, allowing participating centres to maintain ownership of their own data, without introducing duplication and data integrity issues.
DOI: 10.3201/eid1004.030247
发表时间: 2004-04
影响因子: 11.8
作者:
Russell JE;Jolley KA;Feavers IM;Maiden MC;Suker J
通讯作者: Suker J
DOI: 10.1093/bioinformatics/17.11.1077
发表时间: 2001-11-01
期刊: BIOINFORMATICS
影响因子: 5.8
作者:
Chan, MS;Maiden, MCJ;Spratt, BG
通讯作者: Spratt, BG
DOI: 10.1128/jcm.39.1.14-23.2001
发表时间: 2001-01-01
影响因子: 9.4
作者:
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通讯作者: Maiden, MCJ
DOI: 10.1073/pnas.95.6.3140
发表时间: 1998-03-17
影响因子: 11.1
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通讯作者: Spratt, BG
DOI: 10.1128/jcm.38.12.4492-4498.2000
发表时间: 2000-12-01
影响因子: 9.4
作者:
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通讯作者: Maiden, MCJ