Copper-Induced Spermatozoa Head Malformation Is Related to Oxidative Damage to Testes in CD-1 Mice
Copper-Induced Spermatozoa Head Malformation Is Related to Oxidative Damage to Testes in CD-1 Mice
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铜诱导的精子头部畸形与 CD-1 小鼠睾丸氧化损伤有关
DOI：
10.1007/s12011-016-0675-6
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Ying Li
中科院分区：
文献类型：
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作者：
Zhuo-Wei Zhang;Zhi-GangTan;Na Qiao;Zhen-Long Kang;Zhi-Ling Chen;Lian-Mei Hu;Zeng-Ming Yang;Ying Li
铜毒对小鼠精子质量影响的分子机制尚不清楚。在为期4周的实验中，我们对24只6周龄的雄性CD-1小鼠进行了每周两次的腹腔注射氯化铜，并评估了精子质量、睾丸中的铜水平、血清睾酮、关键抗氧化剂谷胱甘肽过氧化物酶5(GPx5)的表达以及小鼠睾丸中调节的雄激素受体(AR)。我们比较了四组小鼠的结果，每组六只小鼠，分别给予0，1.25，2.5，5.0 mg/kg体重的氯化铜，每周两次，持续4周。小鼠表现出铜增加精子头部畸形的剂量反应方式。然而，我们没有观察到精子存活率和顶体完整性在小鼠体重与睾丸重量的比率中的变化，也没有随着平均铜水平的增加而改变睾丸的组织形态。RT-PCR法、免疫组织化学、ELISA法和组织化学分析结果表明，随着铜剂量的增加，睾丸GPx5表达增加，AR表达减少，血清睾酮水平降低，3-β-羟基类固醇脱氢酶活性降低。综上所述，这些数据表明，亚致死性铜暴露可诱发精子头部畸形，并影响睾丸中与铜有关的GPx5和AR的mRNA和蛋白水平。
The molecular mechanism for copper toxicity on spermatozoa quality in mice is not well understood. In a 4-week experiment, we challenged 24, 6-week-old male CD-1 mice with twice-a-week intraperitoneal copper chloride injections and evaluated spermatozoa quality, copper levels in the testes, serum testosterone, the expression of key antioxidant glutathione peroxidase 5 (GPx5), and the regulated androgen receptor (AR) in the mice testes. We compared these outcomes for four groups of six mice given doses of 0, 1.25, 2.5, 5.0 mg/kg weight copper chloride twice a week for 4 weeks. The mice demonstrated a copper increase spermatozoa head malformation in a dose-response manner. However, we observed no changes in spermatozoa viability and acrosome integrity in the ratio of mouse body weight to testes weight or in the histomorphology of the testes as the average copper level increased. Results of our RT-PCR assays, immunohistochemical tests, ELISA, and histochemistry analyses indicated that testis GPx5 expression was increased, AR expression in the testes was decreased, serum testosterone was decreased, and the activity of 3β-hydroxysteroid dehydrogenase was decreased as the copper dose increased. In conclusion, these data show that sublethal exposure to copper induces spermatozoa head malformation and influences both mRNA and protein levels of GPx5 and AR which is related to copper resides in the testes.
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影响因子：
2.7
作者：
Agata Miska-Schramm;M. Kruczek;J. Kapusta
通讯作者：
Agata Miska-Schramm;M. Kruczek;J. Kapusta
影响因子：
3.3
作者：
Chabory, E.;Damon, C.;Drevet, J. R.
通讯作者：
Drevet, J. R.
影响因子：
--
作者：
Qing Zhou;Rong Nie;G. Prins;P. Saunders;B. Katzenellenbogen;R. Hess
通讯作者：
Qing Zhou;Rong Nie;G. Prins;P. Saunders;B. Katzenellenbogen;R. Hess
DOI：
10.1016/j.beem.2015.04.006
发表时间：
2015-08-01
影响因子：
7.4
作者：
O'Hara, Laura;Smith, Lee B.
通讯作者：
Smith, Lee B.
DOI：
--
发表时间：
2013
期刊：
--
影响因子：
--
作者：
通讯作者：
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