Phylogeny and genetic diversity of the banana Fusarium wilt pathogen Fusarium oxysporum f. sp. cubense in the Indonesian centre of origin.
Phylogeny and genetic diversity of the banana Fusarium wilt pathogen Fusarium oxysporum f. sp. cubense in the Indonesian centre of origin.
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DOI：
10.1016/j.simyco.2018.06.003
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发表时间：
2019-03
影响因子：
16.5
通讯作者：
Kema GHJ
中科院分区：
文献类型：
--
作者：
Maryani N;Lombard L;Poerba YS;Subandiyah S;Crous PW;Kema GHJ
尖孢镰刀菌 f. sp。 cubense (Foc) 是香蕉枯萎病或巴拿马病的病原体，是全球香蕉生产的主要限制因素之一。印度尼西亚是野生和栽培香蕉的起源中心，这些香蕉很可能与 Foc 共同进化。这项研究通过在印度尼西亚 6 个岛屿 15 个省的 34 个地理和环境不同的地点进行采样，探索了 Foc 最广泛的遗传多样性。这导致从 40 个不同的当地香蕉品种中全面收集了约 200 个分离株。使用翻译延伸因子-1α (tef1)、RNA 聚合酶 II 最大亚基 (rpb1) 和 RNA 聚合酶 II 第二大亚基 (rpb2) 的序列分析来鉴定和评估分离株。通过对这些基因进行系统发育分析，鉴定出 180 个尖孢镰刀菌 (Fusarium oxysporum f) 分离株。 sp。 cubense (Foc) 和 Fujikuroi 物种复合体 (FFSC)、incarnatum-equiseti 物种复合体 (FIESC) 和接骨木镰刀菌物种复合体 (FSSC) 的 20 个分离株。进一步分析，结合全球范围内的 Foc 菌株，揭示了 Foc 的 9 个独立遗传谱系，以及尖孢镰刀菌物种复合体 (FOSC) 中的一个新分支。从每个谱系中选出的分离株在香蕉品种 Gros Michel 和 Cavendish 上进行了测试，以表征其致病性特征。由于对卡文迪什香蕉具有致病性，超过 65% 的分离株被诊断为热带小种 4 (Foc-TR4)，这支持了 Foc-TR4 源自印度尼西亚的假设。这项研究正式描述了 Foc 的九个独立遗传谱系。自 1919 年首次描述 Foc 以来，尚未对这种生物多样性进行过研究。这项研究详细概述了香蕉枯萎病的复杂性及其在印度尼西亚的多样性和分布。
Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt or Panama disease on banana, is one of the major constraints in banana production worldwide. Indonesia is the centre of origin for wild and cultivated bananas, which likely co-evolved with Foc. This study explored the widest possible genetic diversity of Foc by sampling across Indonesia at 34 geographically and environmentally different locations in 15 provinces at six islands. This resulted in a comprehensive collection of ∼200 isolates from 40 different local banana varieties. Isolates were identified and assessed using sequence analysis of the translation elongation factor-1alpha (tef1), the RNA polymerase II largest subunit (rpb1), and the RNA polymerase II second largest subunit (rpb2). Phylogenetic analyses of these genes allowed the identification of 180 isolates of Fusarium oxysporum f. sp. cubense (Foc), and 20 isolates of the Fusarium fujikuroi species complex (FFSC), the Fusarium incarnatum-equiseti species complex (FIESC), and the Fusarium sambucinum species complex (FSSC). Further analyses, incorporating a worldwide collection of Foc strains, revealed nine independent genetic lineages for Foc, and one novel clade in the Fusarium oxysporum species complex (FOSC). Selected isolates from each lineage were tested on the banana varieties Gros Michel and Cavendish to characterise their pathogenicity profiles. More than 65 % of the isolates were diagnosed as Tropical Race 4 (Foc-TR4) due to their pathogenicity to Cavendish banana, which supports the hypothesis that Foc-TR4 is of Indonesian origin. Nine independent genetic lineages for Foc are formally described in this study. This biodiversity has not been studied since the initial description of Foc in 1919. This study provides a detailed overview of the complexity of Fusarium wilt on banana and its diversity and distribution across Indonesia.
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作者：
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通讯作者：
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