Biodegradation of Tetrahydrofuran by the Newly Isolated Filamentous Fungus Pseudallescheria boydii ZM01
Biodegradation of Tetrahydrofuran by the Newly Isolated Filamentous Fungus Pseudallescheria boydii ZM01
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新分离的丝状真菌 Pseudallescheria boydii ZM01 对四氢呋喃的生物降解
DOI：
10.3390/microorganisms8081190
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发表时间：
2020-08
期刊：
影响因子：
4.5
通讯作者：
Zhenmei Lu
中科院分区：
文献类型：
--
作者：
Hao Ren;Hanbo Li;Haixia Wang;Hui Huang;Zhenmei Lu
四氢呋喃 (THF) 广泛用作聚合物合成的前体和工业中的多功能溶剂。 THF 是一种环境危害，对人类具有致癌性。在本研究中，分离并鉴定了一种新的 THF 降解丝状真菌 Pseudallescheria boydii ZM01。菌株ZM01可耐受的最大THF浓度为260 mM，可在48 h内完全降解5 mM THF，最大THF降解率为133.40 mg THF h−1 g−1干重。当THF初始浓度低于150 mM时，未观察到生长抑制，在50 mM THF下，最大THF降解率仍保持在118.21 mg THF h−1 g−1干重，表明该菌株在高浓度THF降解方面具有巨大潜力。在THF降解过程中，首次通过气相色谱（GC）分析检测并鉴定了初始关键代谢中间体2-羟基四氢呋喃。分析初始pH、培养温度和重金属离子对THF降解的影响表明，菌株ZM01可以在较宽的条件下降解THF，并且在低pH和Cu2+胁迫下具有良好的降解能力，表明其在工业废水处理中的适应性和适用性。
Tetrahydrofuran (THF) is widely used as a precursor for polymer syntheses and a versatile solvent in industries. THF is an environmental hazard and carcinogenic to humans. In the present study, a new THF-degrading filamentous fungus, Pseudallescheria boydii ZM01, was isolated and characterized. Strain ZM01 can tolerate a maximum THF concentration of 260 mM and can completely degrade 5 mM THF in 48 h, with a maximum THF degradation rate of 133.40 mg THF h−1 g−1 dry weight. Growth inhibition was not observed when the initial THF concentration was below 150 mM, and the maximum THF degradation rate was still maintained at 118.21 mg THF h−1 g−1 dry weight at 50 mM THF, indicating the great potential of this strain to degrade THF at high concentrations. The initial key metabolic intermediate 2-hydroxytetrahydrofuran was detected and identified by gas chromatography (GC) analyses for the first time during the THF degradation process. Analyses of the effects of initial pH, incubation temperature, and heavy metal ions on THF degradation revealed that strain ZM01 can degrade THF under a relatively wide range of conditions and has good degradation ability under low pH and Cu2+ stress, suggesting its adaptability and applicability for industrial wastewater treatment.
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