The widespread dissemination of integrons throughout bacterial communities in a riverine system.
The widespread dissemination of integrons throughout bacterial communities in a riverine system.
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DOI：
10.1038/s41396-017-0030-8
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Wellington EMH
中科院分区：
文献类型：
--
作者：
Amos GCA;Ploumakis S;Zhang L;Hawkey PM;Gaze WH;Wellington EMH
人为输入增加了环境中的抗菌素耐药性（AMR）水平，然而，尚不清楚这些输入如何导致这种观察到的增加，以及人为来源是否影响环境细菌的AMR。本研究的目的是探讨污水处理厂（WWTPs）在河流环境中传播1类整合子（CL 1）的作用。使用样本网站从上游和下游的污水处理厂，我们通过隔离和文化独立的分析表明，污水处理厂的污水显着增加CL 1丰度和抗生素耐药性在河流环境。对CL 1分离株的表征表明，CL 1分布在各种细菌中，具有相同的复杂遗传耐药决定因素，这些决定因素从人类相关细菌和常见的环境细菌中分离出来。超过一半的测序CL 1 s缺乏3′-保守序列（“非典型”CL 1 s）;令人惊讶的是，携带非典型CL 1 s的细菌平均比携带3′-CS CL 1 s的细菌对更多的抗生素耐药。季铵化合物（QAC）的耐药基因在75%的测序CL 1基因盒阵列。化学数据分析表明，污水处理厂下游的硼（一种洗涤剂标志物）含量很高。随后对携带CL 1的分离株进行的表型筛选表明，约90%的分离株对QAC去污剂具有抗性，体外实验表明，QAC可以单独选择将临床抗生素抗性基因转移至未处理的大肠杆菌受体。总之，本研究强调了污水处理厂对环境AMR的重大影响，并证明了环境相关细菌对临床重要耐药决定因素的广泛携带。
Anthropogenic inputs increase levels of antimicrobial resistance (AMR) in the environment, however, it is unknown how these inputs create this observed increase, and if anthropogenic sources impact AMR in environmental bacteria. The aim of this study was to characterise the role of waste water treatment plants (WWTPs) in the dissemination of class 1 integrons (CL1s) in the riverine environment. Using sample sites from upstream and downstream of a WWTP, we demonstrate through isolation and culture-independent analysis that WWTP effluent significantly increases both CL1 abundance and antibiotic resistance in the riverine environment. Characterisation of CL1-bearing isolates revealed that CL1s were distributed across a diverse range of bacteria, with identical complex genetic resistance determinants isolated from both human-associated and common environmental bacteria across connected sites. Over half of sequenced CL1s lacked the 3′-conserved sequence ('atypical’ CL1s); surprisingly, bacteria carrying atypical CL1s were on average resistant to more antibiotics than bacteria carrying 3′-CS CL1s. Quaternary ammonium compound (QAC) resistance genes were observed across 75% of sequenced CL1 gene cassette arrays. Chemical data analysis indicated high levels of boron (a detergent marker) downstream of the WWTP. Subsequent phenotypic screening of CL1-bearing isolates demonstrated that ~90% were resistant to QAC detergents, with in vitro experiments demonstrating that QACs could solely select for the transfer of clinical antibiotic resistance genes to a naive Escherichia coli recipient. In conclusion, this study highlights the significant impact of WWTPs on environmental AMR, and demonstrates the widespread carriage of clinically important resistance determinants by environmentally associated bacteria.
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DOI：
10.1016/j.diagmicrobio.2006.12.019
发表时间：
2007-06-01
影响因子：
2.9
作者：
Nhung, Pham Hong;Ohkusu, Kiyofumi;Ezaki, Takayuki
通讯作者：
Ezaki, Takayuki
河水和沉积物中的1类整合子积聚酶和磺酰胺耐药基因受人为压力和环境因素的影响。
影响因子：
3.6
作者：
Koczura, Ryszard;Mokracka, Joanna;Taraszewska, Agata;Lopacinska, Natalia
通讯作者：
Lopacinska, Natalia
影响因子：
4.9
作者：
PAULSEN, IT;LITTLEJOHN, TG;SKURRAY, RA
通讯作者：
SKURRAY, RA
影响因子：
11.8
作者：
Leonard, Anne F. C.;Zhang, Lihong;Gaze, William H.
通讯作者：
Gaze, William H.
影响因子：
3.3
作者：
Amos, G. C. A.;Zhang, L.;Wellington, E. M.
通讯作者：
Wellington, E. M.