Members of the tomato FRUITFULL MADS-box family regulate style abscission and fruit ripening.
Members of the tomato FRUITFULL MADS-box family regulate style abscission and fruit ripening.
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番茄 FRUITFULL MADS-box 家族的成员调节花柱脱落和果实成熟
DOI：
10.1093/jxb/eru137
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发表时间：
2014-07
影响因子：
6.9
通讯作者：
Ye Z
中科院分区：
文献类型：
--
作者：
Wang S;Lu G;Hou Z;Luo Z;Wang T;Li H;Zhang J;Ye Z
FRUITFULL基因以冗余方式调节番茄果实成熟。它们通过影响细胞的分化和扩展而在果形调节中发挥功能性作用。番茄（Solanum lycopersicum）蛋白MADS-RIN在果实成熟中起重要作用。在这项研究中，功能的两个同源番茄蛋白，FUL 1和FUL 2，其中包含保守的MIKC结构域，代表植物MADS-box蛋白，并与MADS-RIN相互作用，进行了分析。转基因功能分析表明，FUL 1和FUL 2在果实成熟调控中具有冗余功能，但在细胞分化和扩展调控中表现出不同的作用。FUL 2基因在番茄中的过表达导致果实开花端出现尖形，果皮变薄，茎直径变小，叶片变小，但FUL 1基因的过表达没有引起明显的表型变化。FUL 1和FUL 2的双重抑制通过阻断乙烯生物合成和减少类胡萝卜素积累而显著抑制果实成熟。此外，对应于ACC SYNTHASE 2（ACS 2），这在乙烯生物合成中起着关键作用的转录物的水平显着降低，在FUL 1/FUL 2敲低番茄果实。总之，我们的研究结果表明，FUL蛋白可以通过微调乙烯生物合成和成熟相关基因的表达来调节番茄果实成熟。
The FRUITFULL genes regulate tomato fruit ripening in a redundant manner. They are functional diversely in fruit shape regulation through affecting cellular differentiation and expansion. The tomato (Solanum lycopersicum) protein MADS-RIN plays important roles in fruit ripening. In this study, the functions of two homologous tomato proteins, FUL1 and FUL2, which contain conserved MIKC domains that typify plant MADS-box proteins, and which interact with MADS-RIN, were analysed. Transgenic functional analysis showed that FUL1 and FUL2 function redundantly in fruit ripening regulation, but exhibit distinct roles in the regulation of cellular differentiation and expansion. Over-expression of FUL2 in tomato resulted in a pointed tip at the blossom end of the fruit, together with a thinner pericarp, reduced stem diameter, and smaller leaves, but no obvious phenotypes resulted from FUL1 over-expression. Dual suppression of FUL1 and FUL2 substantially inhibited fruit ripening by blocking ethylene biosynthesis and decreasing carotenoid accumulation. In addition, the levels of transcript corresponding to ACC SYNTHASE2 (ACS2), which plays a key role in ethylene biosynthesis, were significantly decreased in the FUL1/FUL2 knock-down tomato fruits. Overall, our results suggest that FUL proteins can regulate tomato fruit ripening through fine-tuning ethylene biosynthesis and the expression of ripening-related genes.
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