Development of 3D bioprinting: From printing methods to biomedical applications.

Development of 3D bioprinting: From printing methods to biomedical applications.
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3D生物打印的开发:从印刷方法到生物医学应用。

DOI:
10.1016/j.ajps.2019.11.003
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发表时间:
2020-09
影响因子:
10.2
通讯作者:
He Y
He Y
中科院分区:
医学1区
文献类型:
--
作者:
Gu Z;Fu J;Lin H;He Y

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体外组织/器官的生物制造是我们的大梦想,这是由两个需求驱动的:器官移植和准确的组织模型。在过去的几十年里,3D生物打印已经被广泛应用于皮肤、血管、心脏等许多组织/器官的构建,不仅可以为器官替代的宏伟目标奠定基础,还可以作为致力于药代动力学、药物筛选等的体外模型。由于器官如此复杂,许多生物打印方法被用来解决不同应用的挑战。那么问题是如何选择合适的生物印花方法呢?在此,我们系统地回顾了3D生物打印的发展、过程和分类,重点介绍了3D生物打印的基本原理和商业化的生物打印机。对挤压印花、液滴印花和光固化印花三种生物印花方法进行了总结和分类,并提出了应用建议。其中重点介绍了同轴生物印刷和多材料生物印刷,并讨论了生物油墨设计的基本原则。
Biomanufacturing of tissues/organs in vitro is our big dream, driven by two needs: organ transplantation and accurate tissue models. Over the last decades, 3D bioprinting has been widely applied in the construction of many tissues/organs such as skins, vessels, hearts, etc., which can not only lay a foundation for the grand goal of organ replacement, but also be served as in vitro models committed to pharmacokinetics, drug screening and so on. As organs are so complicated, many bioprinting methods are exploited to figure out the challenges of different applications. So the question is how to choose the suitable bioprinting method? Herein, we systematically review the evolution, process and classification of 3D bioprinting with an emphasis on the fundamental printing principles and commercialized bioprinters. We summarize and classify extrusion-based, droplet-based, and photocuring-based bioprinting methods and give some advices for applications. Among them, coaxial and multi-material bioprinting are highlighted and basic principles of designing bioinks are also discussed.
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