Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection.
Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection.
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DOI：
10.1021/acsomega.2c08127
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发表时间：
2023-03-07
期刊：
影响因子：
4.1
通讯作者：
Leese, Hannah S.
中科院分区：
文献类型：
--
作者：
Mustafa, Yasemin L.;Leese, Hannah S.
开发用于靶向生物标志物检测的灵敏和选择性的强健传感器材料，旨在促进自我健康监测和管理。分子印迹聚合物(MIP)材料可以作为仿生识别元件，通过定制的合成路线进行特定目标分析物的提取和/或检测。本工作以甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂，合成了一种灵敏、选择性的乳酸分子印迹聚合物。通过测定目标分析物保留率、印迹因子和选择性吸附分别达到63.5%、6.86和0.82来评价合成的印迹物种的灵敏度。MIP选择性阐明了功能单体与目标分析物乳酸之间的印迹机理，并通过结构竞争性分析物苹果酸和2-羟基丁酸钠的实验进一步证实，它们的保留率分别为22.6%和25.2%。了解模板分析物和结构干扰物与MIP的特定分子间机制使实验者能够就单体-目标和致孔剂的选择以及改善分子印迹的可能相互作用位置做出明智的决定。这种印迹系统突出了进一步发展成为用于检测疾病的人造受体传感器材料的潜力。
The development of sensitive and selective robust sensor materials for targeted biomarker detection aims to contribute to self-health monitoring and management. Molecularly imprinted polymeric (MIP) materials can perform as biomimetic recognition elements via tailored routes of synthesis for specific target analyte extraction and/or detection. In this work, a sensitive- and selective-lactate MIP has been developed utilizing methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and cross-linker, respectively. The sensitivity of the as-synthesized imprinted species was evaluated by determining the target analyte retention, imprinting factor, and selectivity adsorption of up to 63.5%, 6.86, and 0.82, respectively. MIP selectivity elucidated the imprinting mechanism between the functional monomers and target analyte lactate, further experimentally evidenced by using structurally competitive analytes malic acid and sodium 2-hydroxybutyrate, where retentions of 22.6 and 25.2%, respectively, were observed. Understanding the specific intermolecular mechanisms of both the template analyte and structural interferents with the MIP enables experimentalists to make informed decisions regarding monomer-target and porogen selections and possible sites of interaction for improved molecular imprinting. This imprinting system highlights the potential to be further developed into artificial receptor sensor materials for the detection of disease.
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影响因子：
12.6
作者：
Athikomrattanakul, Umporn;Katterle, Martin;Scheller, Frieder W.
通讯作者：
Scheller, Frieder W.
影响因子：
4.7
作者：
Daniels, Emma;Mustafa, Yasemin L.;Leese, Hannah S.
通讯作者：
Leese, Hannah S.
表面活性剂介导的溶胶-凝胶法制备分子印迹聚合物及其在仿生免疫分析中检测蛋白质的应用
DOI：
10.1016/j.jpba.2020.113511
发表时间：
2020-10-25
影响因子：
3.4
作者：
Chen Guoning;Shu Hua;Fu Qiang
通讯作者：
Fu Qiang
影响因子：
3.5
作者：
Gomes, Catarina;Sadoyan, Gayane;Costa, Mario Rui P. F. N.
通讯作者：
Costa, Mario Rui P. F. N.
影响因子：
7.4
作者：
Lanza, F;Sellergren, B
通讯作者：
Sellergren, B