Efficacy of extracellular vesicles from dental pulp stem cells for bone regeneration in rat calvarial bone defects.
Efficacy of extracellular vesicles from dental pulp stem cells for bone regeneration in rat calvarial bone defects.
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DOI：
10.1186/s41232-021-00163-w
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发表时间：
2021-04-14
影响因子：
8.1
通讯作者：
Takebe J
中科院分区：
文献类型：
--
作者：
Imanishi Y;Hata M;Matsukawa R;Aoyagi A;Omi M;Mizutani M;Naruse K;Ozawa S;Honda M;Matsubara T;Takebe J
已知细胞外囊泡（EV）由各种细胞分泌。特别地，间充质干细胞（MSC）衍生的EV（MSC-EV）具有组织修复能力和抗炎特性。牙髓干细胞（DPSC）是从牙髓组织中分离的MSC，比其他MSC对身体的侵袭性更小，并且可以从年轻个体中收集。在这项研究中，我们研究了由DPSC分泌的EV（DPSC-EV）用于骨形成的功效。从DPSC的细胞培养基中分离DPSC-EV。将DPSC-EV与胶原（COL）、β-磷酸三钙（β-TCP）或羟基磷灰石（HA）一起沿着注入大鼠颅骨缺损。通过显微CT和组织学观察分析DPSC-EV的作用。Micro-CT显示，给予具有上述支架的DPSC-EV导致在缺损周边的骨形成。DPSC-EV/COL特别导致缺损中心的骨形成。组织学观察显示DPSC-EVs/COL促进新骨形成。DPSC-EVs/COL对骨缺损部位的作用与DPSC/COL移植几乎相同。这些结果表明，DPSC-EVs可能是骨组织再生的有效工具。
Extracellular vesicles (EVs) are known to be secreted by various cells. In particular, mesenchymal stem cell (MSC)-derived EVs (MSC-EVs) have tissue repair capacity and anti-inflammatory properties. Dental pulp stem cells (DPSCs), which are MSCs isolated from pulp tissue, are less invasive to the body than other MSCs and can be collected from young individuals. In this study, we investigated the efficacy of EVs secreted by DPSCs (DPSC-EVs) for bone formation. DPSC-EVs were isolated from the cell culture medium of DPSCs. DPSC-EVs were unilaterally injected along with collagen (COL), beta-tricalcium phosphate (β-TCP) or hydroxyapatite (HA) into rat calvarial bone defects. The effects of DPSC-EVs were analyzed by micro-computed tomography (micro-CT) and histological observation. Micro-CT showed that administration of DPSC-EVs with the abovementioned scaffolds resulted in bone formation in the periphery of the defects. DPSC-EVs/COL specifically resulted in bone formation in the center of the defects. Histological observation revealed that DPSC-EVs/COL promoted new bone formation. Administration of DPSC-EVs/COL had almost the same effect on the bone defect site as transplantation of DPSCs/COL. These results suggest that DPSC-EVs may be effective tools for bone tissue regeneration.
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影响因子：
3
作者：
Valdivia-Gandur I;Engelke W;Beltrán V;Borie E;Fuentes R;Manzanares-Céspedes MC
通讯作者：
Manzanares-Céspedes MC
影响因子：
2.6
作者：
Bartholomew, A;Sturgeon, C;Hoffman, R
通讯作者：
Hoffman, R
DOI：
10.1038/nri3622
发表时间：
2014-03
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
0.4
作者：
Matsukawa, Ryohei;Ozawa, Shogo;Tanaka, Yoshinobu
通讯作者：
Tanaka, Yoshinobu
外泌体在氧化应激期间传达保护性信息；外泌体穿梭RNA的可能作用。
影响因子：
3.7
作者：
Eldh M;Ekström K;Valadi H;Sjöstrand M;Olsson B;Jernås M;Lötvall J
通讯作者：
Lötvall J