Suppressed monocyte gene expression profile in men versus women with PTSD.
Suppressed monocyte gene expression profile in men versus women with PTSD.
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DOI：
10.1016/j.bbi.2010.12.001
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发表时间：
2011-03
影响因子：
15.1
通讯作者：
Pulliam, Lynn
中科院分区：
文献类型：
--
作者：
Neylan, Thomas C.;Sun, Bing;Rempel, Hans;Ross, Jessica;Lenoci, Maryann;O'Donovan, Aoife;Pulliam, Lynn
已经有几次尝试使用来自外周血单核细胞的基因微阵列来鉴定创伤后应激障碍（PTSD）治疗的新生物学途径或靶点。迄今为止进行的几项研究产生了一种不明确的发现模式，这可能反映了使用循环免疫细胞的异质样本进行分析。我们在循环单核细胞的同质样本上使用基因微阵列来测试慢性创伤后应激障碍与炎症活动升高相关的假设，并识别该疾病中失调的新途径。招募了49名男性（24名PTSD+和25名年龄匹配的创伤暴露PTSD-对照）和18名女性（10名PTSD+和8名年龄匹配的PTSD-对照）。在CD 14+单核细胞上进行基因表达微阵列分析，所述单核细胞是启动和响应炎症信号传导的免疫细胞。患有PTSD的男性受试者具有单核细胞上基因表达不足的总体模式（47个表达不足对4个过度表达基因）。多重比较的严格校正和q-PCR验证表明，只有3个基因的差异表达，都是低表达。在男性PTSD+受试者中没有慢性炎症的转录证据。相比之下，我们的飞行员女性PTSD+受试者的初步数据显示，基因表达增加和减少以及与免疫激活相关的途径活性增加的模式相对平衡。结果表明PTSD单核细胞基因表达的差异模式，从我们的女性飞行员科目的初步数据表明，性别二型性的生物通路激活PTSD。免疫细胞基因表达的变化可能导致PTSD的医学发病率。
There have been several attempts to use gene microarrays from peripheral blood mononuclear cells to identify new biological pathways or targets for therapy in Posttraumatic Stress Disorder (PTSD). The few studies conducted to date have yielded an unclear pattern of findings, perhaps reflecting the use of heterogeneous samples of circulating immune cells for analysis. We used gene microarrays on a homogeneous sample of circulating monocytes to test the hypothesis that chronic PTSD would be associated with elevated inflammatory activity and to identify new pathways dysregulated in the disorder. Forty-nine men (24 PTSD+ and 25 age-matched trauma-exposed PTSD− controls) and 18 women (10 PTSD+ and 8 age-matched PTSD− controls) were recruited. Gene expression microarray analysis was performed on CD14+ monocytes, immune cells that initiate and respond to inflammatory signaling. Male subjects with PTSD had an overall pattern of under-expression of genes on monocytes (47 under-expressed versus 4 over-expressed genes). A rigorous correction for multiple comparisons and verification with q-PCR showed that of only 3 genes that were differentially expressed, all were under-expressed. There was no transcriptional evidence of chronic inflammation in male PTSD+ subjects. In contrast, preliminary data from our pilot female PTSD+ subjects showed a relatively balanced pattern of increased and decreased expression of genes and an increase in activity of pathways related to immune activation. The results indicate differential patterns of monocyte gene expression in PTSD, and the preliminary data from our female pilot subjects are suggestive of gender dimorphism in biologic pathways activated in PTSD. Changes in immune cell gene expression may contribute to medical morbidity in PTSD.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
--
作者：
KESSLER, RC;SONNEGA, A;NELSON, CB
通讯作者：
NELSON, CB
影响因子：
7.4
作者：
Hoge, E. A.;Brandstetter, K.;Simon, N. M.
通讯作者：
Simon, N. M.
影响因子：
10.6
作者：
Maes, M;Lin, AH;Bosmans, E
通讯作者：
Bosmans, E
影响因子：
3.8
作者：
Maes, M;Song, C;Smith, RS
通讯作者：
Smith, RS