Short-term observations of the regenerative potential of injured proximal sensory nerves crossed with distal motor nerves.
Short-term observations of the regenerative potential of injured proximal sensory nerves crossed with distal motor nerves.
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受伤的近端感觉神经与远端运动神经交叉的再生潜力的短期观察
DOI：
10.4103/1673-5374.211199
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发表时间：
2017-07
影响因子：
6.1
通讯作者：
Zhang PX
中科院分区：
文献类型：
--
作者：
Zhang XX;Kou YH;Yin XF;Jiang BG;Zhang PX
运动神经和感觉神经以不同的方向传导信号，并以不同的方式发挥作用。在周围神经损伤的外科治疗中，保持运动神经和感觉神经分离并使用缝合法修复神经可获得最佳预后。然而，感觉神经和运动神经之间的连接的临床后果目前仍然未知。本研究分析了大鼠股神经的解剖结构，观察了股神经在股四头肌内的运动支和感觉支。神经结扎后，将感觉神经近端与运动神经远端连接，观察新生神经纤维的变化。乙酰胆碱酯酶染色用于区分有髓和无髓运动和感觉神经。对失神经肌肉和新生神经进行形态学、电生理和组织化学比较。连接后8周，神经导管两侧未见运动神经纤维，近端神经纤维增多。新形成的运动和感觉纤维的比例是不同的两侧的管道。近端再生神经中自主神经所占的面积有限，但远端神经中未见明显的髓鞘。这些结果证实了感觉神经和运动神经不能有效连接。此外，远端靶器官的改变影响近端神经的类型。
Motor nerves and sensory nerves conduct signals in different directions and function in different ways. In the surgical treatment of peripheral nerve injuries, the best prognosis is obtained by keeping the motor and sensory nerves separated and repairing the nerves using the suture method. However, the clinical consequences of connections between sensory and motor nerves currently remain unknown. In this study, we analyzed the anatomical structure of the rat femoral nerve, and observed the motor and sensory branches of the femoral nerve in the quadriceps femoris. After ligation of the nerves, the proximal end of the sensory nerve was connected with the distal end of the motor nerve, followed by observation of the changes in the newly-formed regenerated nerve fibers. Acetylcholinesterase staining was used to distinguish between the myelinated and unmyelinated motor and sensory nerves. Denervated muscle and newly formed nerves were compared in terms of morphology, electrophysiology and histochemistry. At 8 weeks after connection, no motor nerve fibers were observed on either side of the nerve conduit and the number of nerve fibers increased at the proximal end. The proportion of newly-formed motor and sensory fibers was different on both sides of the conduit. The area occupied by autonomic nerves in the proximal regenerative nerve was limited, but no distinct myelin sheath was visible in the distal nerve. These results confirm that sensory and motor nerves cannot be effectively connected. Moreover, the change of target organ at the distal end affects the type of nerves at the proximal end.
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影响因子：
2.5
作者：
WEISS, P;EDDS, MV
通讯作者：
EDDS, MV
影响因子：
64.8
作者：
LOHOF, AM;IP, NY;POO, MM
通讯作者：
POO, MM
影响因子：
3.3
作者：
KUCERA, J;ERNFORS, P;JAENISCH, R
通讯作者：
JAENISCH, R
影响因子：
3.3
作者：
Bain, JR;Veltri, KL;Fahnestock, M
通讯作者：
Fahnestock, M
影响因子：
2.7
作者：
BUNGE, MB;WILLIAMS, AK;WOOD, PM
通讯作者：
WOOD, PM