Expression of clock proteins in developing tooth.
Expression of clock proteins in developing tooth.
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DOI：
10.1016/j.gep.2010.12.002
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Papagerakis P
中科院分区：
文献类型：
--
作者：
Zheng L;Papagerakis S;Schnell SD;Hoogerwerf WA;Papagerakis P
成釉细胞和成牙本质细胞分化过程中的形态和功能变化表明牙釉质和牙本质的形成受到昼夜节律的控制。昼夜节律是一种以 24 小时为周期的内源性自我维持振荡，控制着多种生理和代谢过程。哺乳动物时钟基因在同步许多器官的昼夜节律功能方面发挥着关键作用。然而，对于牙齿发育过程中时钟基因的表达几乎一无所知。在这项工作中，我们研究了牙齿发育过程中四个时钟基因的表达。我们的结果表明，通过 RT-PCR 在牙齿中检测到生物钟基因 Bmal1、clock、per1 和 per2 mRNA。免疫组织化学显示，时钟蛋白表达首先在钟形阶段（E17）的牙齿中检测到，并在 EOE 和牙乳头细胞中表达。在出生后第四天（PN4），所有四种时钟蛋白继续在牙齿中表达，但强度不同，在成釉细胞和成牙本质细胞的细胞核中强烈表达，在牙髓细胞中下调。有趣的是，在 PN21 切牙，时钟蛋白的表达在牙冠类似侧的成牙本质细胞中下调，但在牙根类似侧的成牙本质细胞中表达持续存在。相比之下，牙冠和根部成牙本质细胞在 PN21 的第一磨牙中的所有四种时钟蛋白均被强烈染色。在牙周膜 (PDL) 间隙内，马拉塞兹上皮残基 (ERM) 在其他 PDL 细胞中表现出最强的表达。我们的数据表明，时钟基因可能参与成釉细胞和成牙本质细胞功能的调节，例如牙釉质和牙本质蛋白的分泌和基质矿化。
Morphological and functional changes during ameloblast and odontoblast differentiation suggest that enamel and dentin formation is under circadian control. Circadian rhythms are endogenous self-sustained oscillations with periods of 24 hours that control diverse physiological and metabolic processes. Mammalian clock genes play a key role in synchronizing circadian functions in many organs. However, close to nothing is known on clock genes expression during tooth development. In this work, we investigated the expression of four clock genes during tooth development. Our results showed that circadian clock genes Bmal1, clock, per1, and per2 mRNAs were detected in teeth by RT-PCR. Immunohistochemistry showed that clock protein expression was first detected in teeth at the bell stage (E17), being expressed in EOE and dental papilla cells. At post-natal day four (PN4), all four clock proteins continued to be expressed in teeth but with different intensities, being strongly expressed within the nucleus of ameloblasts and odontoblasts and down-regulated in dental pulp cells. Interestingly, at PN21 incisor, expression of clock proteins was down-regulated in odontoblasts of the crown-analogue side but expression was persisting in root-analogue side odontoblasts. In contrast, both crown and root odontoblasts were strongly stained for all four clock proteins in first molars at PN21. Within the periodontal ligament (PDL) space, epithelial rests of Malassez (ERM) showed the strongest expression among other PDL cells. Our data suggests that clock genes might be involved in the regulation of ameloblast and odontoblast functions, such as enamel and dentin protein secretion and matrix mineralization.
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影响因子：
9.2
作者：
Myers, EM;Yu, JJ;Sehgal, A
通讯作者：
Sehgal, A
影响因子：
16.2
作者：
WELSH, DK;LOGOTHETIS, DE;REPPERT, SM
通讯作者：
REPPERT, SM
影响因子：
7.6
作者：
Ohtsuka, M;Saeki, S;Shinoda, H
通讯作者：
Shinoda, H
通过改良消减杂交法测定参与大鼠磨牙根发育起始早期过程的基因。
DOI：
10.1016/j.bbrc.2007.09.087
发表时间：
2007-11-30
影响因子：
3.1
作者：
Xing, Xianghui;Deng, Zhihong;Jin, Yan
通讯作者：
Jin, Yan
影响因子：
3
作者：
Li, CF;Risnes, S
通讯作者：
Risnes, S