Combined QTL mapping, physiological and transcriptomic analyses to identify candidate genes involved in Brassica napus seed aging
Combined QTL mapping, physiological and transcriptomic analyses to identify candidate genes involved in Brassica napus seed aging
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结合 QTL 作图、生理学和转录组分析来鉴定参与甘蓝型油菜种子老化的候选基因
DOI：
10.1007/s00438-018-1468-8
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发表时间：
2018-07
影响因子：
3.1
通讯作者：
Liu Liezhao
中科院分区：
文献类型：
--
作者：
Wang Tengyue;Hou Lintao;Jian Hongju;Di Feifei;Li Jiana;Liu Liezhao
种子老化是油料作物种质资源保存中不可避免的问题。因此，弄清种子衰老的遗传机制对油菜育种具有重要意义。本研究将甘蓝型油菜种子置于人工老化环境中(40℃、90%相对湿度)。利用172个重组自交系群体，在8条染色体上检测到13个QTL，可解释表型变异的9.05%。在两个环境中发现的QTL q2015AGIA-C08和q2016AGI-C08-2被认为是相同的QTL。人工老化后，种子萌发指数降低，相对电导率、丙二醛和脯氨酸含量增加，可溶性糖、蛋白质含量和抗氧化酶活性降低。此外，对未处理(R0和S0)或人工老化15天(R15和S15)的极端品系的种子进行了转录组测序。R15与R0、S15与S0、R0与S0、R15与S15的差异表达基因分别为2843、1084、429和1055个。通过综合QTL定位和RNA测序分析，筛选出编码热激转录因子C_1的基因B-aA03g37460D和编码磷酸果糖激酶4的基因B-aA-03g40360D等7个与种子衰老有关的候选基因。对这些候选基因的进一步研究可以拓宽我们对种子衰老调控机制的理解。
Seed aging is an inevitable problem in the germplasm conservation of oil crops. Thus, clarifying the genetic mechanism of seed aging is important for rapeseed breeding. In this study, Brassica napus seeds were exposed to an artificial aging environment (40 °C and 90% relative humidity). Using a population of 172 recombinant inbred lines, 13 QTLs were detected on 8 chromosomes, which explained ~ 9.05% of the total phenotypic variation. The QTLs q2015AGIA-C08 and q2016AGI-C08-2 identified in the two environments were considered the same QTL. After artificial aging, lower germination index, increased relative electrical conductivity, malondialdehyde and proline content, and reduced soluble sugar, protein content and antioxidant enzyme activities were detected. Furthermore, seeds of extreme lines that were either left untreated (R0 and S0) or subjected to 15 days of artificial aging (R15 and S15) were used for transcriptome sequencing. In total, 2843, 1084, 429 and 1055 differentially expressed genes were identified in R15 vs. R0, S15 vs. S0, R0 vs. S0 and R15 vs. S15, respectively. Through integrated QTL mapping and RNA-sequencing analyses, seven genes, such as BnaA03g37460D, encoding heat shock transcription factor C1, and BnaA03g40360D, encoding phosphofructokinase 4, were screened as candidate genes involved in seed aging. Further researches on these candidate genes could broaden our understanding of the regulatory mechanisms of seed aging.
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影响因子：
13.8
作者：
Lee J;Welti R;Roth M;Schapaugh WT;Li J;Trick HN
通讯作者：
Trick HN
影响因子：
7.4
作者：
Beisson, F;Koo, AJK;Ohlrogge, JB
通讯作者：
Ohlrogge, JB
影响因子：
7.4
作者：
SCOTT, TA;MELVIN, EH
通讯作者：
MELVIN, EH
影响因子：
1.4
作者：
Freitas, R. A.;Dias, D. C. F. S.;Jose, I. C.
通讯作者：
Jose, I. C.
DOI：
10.1017/s1479262111000372
发表时间：
2011-07-01
影响因子：
1.1
作者：
Nagel, Manuela;Rosenhauer, Maria;Boerner, Andreas
通讯作者：
Boerner, Andreas