High-Density Genetic Map Construction and Identification of QTLs Controlling Leaf Abscission Trait in Poncirus trifoliata.
High-Density Genetic Map Construction and Identification of QTLs Controlling Leaf Abscission Trait in Poncirus trifoliata.
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DOI：
10.3390/ijms22115723
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发表时间：
2021-05-27
影响因子：
5.6
通讯作者：
Hu CG
中科院分区：
文献类型：
--
作者：
Xu YY;Liu SR;Gan ZM;Zeng RF;Zhang JZ;Hu CG
高密度遗传连锁图对于包括QTL定位、基因组组装和比较基因组分析在内的遗传和基因组研究是必不可少的。在这里，我们构建了一个柑橘高密度连锁图谱，利用SSR和SNP标记，这是均匀分布在柑橘基因组。该连锁图包含4163个标记，平均距离为1.12cM。该连锁图包含1478个标记，遗传长度分别为1093.90 cM和1227.03 cM。同时，遗传图谱的比较表明，共同标记的线性顺序在柑橘和枳之间高度保守。基于这张高密度整合的柑橘遗传图谱和两年的落叶表型数据，利用关联分析在scaffold 1（包含36个基因）和scaffold 8（包含107个基因）上检测到2个影响叶片脱落表型变异的位点。此外，30个候选基因的表达模式进行了研究，在冷胁迫条件下，因为寒冷的温度是密切相关的落叶性状。该图谱的构建将为柑橘落叶性状的QTL定位和基因组学研究提供基础，同时对柑橘落叶性状的定位研究也将为柑橘落叶性状的遗传机制和柑橘育种提供参考。
A high-density genetic linkage map is essential for genetic and genomic studies including QTL mapping, genome assembly, and comparative genomic analysis. Here, we constructed a citrus high-density linkage map using SSR and SNP markers, which are evenly distributed across the citrus genome. The integrated linkage map contains 4163 markers with an average distance of 1.12 cM. The female and male linkage maps contain 1478 and 2976 markers with genetic lengths of 1093.90 cM and 1227.03 cM, respectively. Meanwhile, a genetic map comparison demonstrates that the linear order of common markers is highly conserved between the clementine mandarin and Poncirus trifoliata. Based on this high-density integrated citrus genetic map and two years of deciduous phenotypic data, two loci conferring leaf abscission phenotypic variation were detected on scaffold 1 (including 36 genes) and scaffold 8 (including 107 genes) using association analysis. Moreover, the expression patterns of 30 candidate genes were investigated under cold stress conditions because cold temperature is closely linked with the deciduous trait. The developed high-density genetic map will facilitate QTL mapping and genomic studies, and the localization of the leaf abscission deciduous trait will be valuable for understanding the mechanism of this deciduous trait and citrus breeding.
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影响因子：
6.9
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
影响因子：
5.3
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
柑橘与柑橘遗传变异及基因交换的鉴定
影响因子：
5.5
作者：
Liu, Tian-Jia;Zhou, Jing-Jing;Hu, Chun-Gen
通讯作者：
Hu, Chun-Gen
通过全基因组分析开发和鉴定柑橘基因组和表达的 SSR
影响因子：
3.7
作者：
Liu SR;Li WY;Long D;Hu CG;Zhang JZ
通讯作者：
Zhang JZ
影响因子：
3.1
作者：
Bernet, G. P.;Fernandez-Ribacoba, J.;Asins, M. J.
通讯作者：
Asins, M. J.