Prevalence of Vector-Borne Pathogens in Reproductive and Non-Reproductive Tissue Samples from Free-Roaming Domestic Cats in the South Atlantic USA.
Prevalence of Vector-Borne Pathogens in Reproductive and Non-Reproductive Tissue Samples from Free-Roaming Domestic Cats in the South Atlantic USA.
复制标题
美国南大西洋自由漫游家猫的生殖和非生殖组织样本中媒介传播病原体的流行情况。
DOI：
10.3390/pathogens10091221
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发表时间：
2021-09-21
期刊：
影响因子：
--
通讯作者：
Lashnits E
中科院分区：
文献类型：
--
作者：
Manvell C;Ferris K;Maggi R;Breitschwerdt EB;Lashnits E
自由漫游猫 (FRC) 是多种人畜共患病原体的宿主，与家养和野生动物、病媒和人类相互作用。为了评估猫媒介传播的病原体垂直传播的可能性，本研究调查了美国南大西洋两个地点 FRC 的耳尖和生殖组织中的无形体、巴尔通体、埃利希体、嗜血支原体和立克次体。我们收集了 73 只猫的卵巢 (n = 72)、子宫 (n = 54)、睾丸 (n = 74) 和耳尖 (n = 73) 组织，以及 11 只猫王的胎儿 (n = 20) 和胎盘 (n = 19) 组织。利用 qPCR 扩增病原体 DNA，并通过测序确认。猫的生殖组织（19%，14/73）比耳尖（5%，4/73；p = 0.02）更容易出现汉赛巴尔通体阳性。 B. henselae 从胎儿 (20%, 4/20) 和胎盘样本 (11%, 2/19) 中扩增。巴尔通体属北卡罗来纳州的猫（76%，26/34）感染比弗吉尼亚州的猫（13%，5/39；p < 0.0001）更常见。百分之十四 (10/73) 的耳尖和生殖组织均呈嗜血性支原体阳性。无形体、埃利希体和立克次体。任何猫/组织的 DNA 均未扩增。这些发现表明，汉赛博德菌优先感染猫的生殖组织，并强调了研究汉赛博德菌垂直传播或诱导生殖失败的可能性的重要性。
Reservoir to multiple species of zoonotic pathogens, free-roaming cats (FRCs) interact with domestic and wild animals, vectors, and humans. To assess the potential for feline vector-borne pathogens to be vertically transmitted, this study surveyed ear tip and reproductive tissues of FRCs from two locations in the South Atlantic United States for Anaplasma, Bartonella, Ehrlichia, hemotropic Mycoplasma, and Rickettsia species. We collected ovary (n = 72), uterus (n = 54), testicle (n = 74), and ear tip (n = 73) tissue from 73 cats, and fetal (n = 20) and placental (n = 19) tissue from 11 queens. Pathogen DNA was amplified utilizing qPCR, confirmed by sequencing. Cats were more frequently Bartonella henselae positive on reproductive tissues (19%, 14/73) than ear tip (5%, 4/73; p = 0.02). B. henselae was amplified from fetus (20%, 4/20) and placenta samples (11%, 2/19). Bartonella spp. infection was more common in cats from North Carolina (76%, 26/34) than Virginia (13%, 5/39; p < 0.0001). Fourteen percent (10/73) of both ear tip and reproductive tissues were positive for hemotropic Mycoplasma spp. Anaplasma, Ehrlichia, and Rickettsia spp. DNA was not amplified from any cat/tissue. These findings suggest that B. henselae preferentially infected cats’ reproductive tissue and reinforces the importance of investigating the potential for B. henselae vertical transmission or induction of reproductive failure.
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影响因子：
9.4
作者：
Birkenheuer, AJ;Levy, MG;Breitschwerdt, EB
通讯作者：
Breitschwerdt, EB
影响因子：
3.3
作者：
Fleischman, Drew A.;Chomel, Bruno B.;Diniz, Pedro Paulo V. P.
通讯作者：
Diniz, Pedro Paulo V. P.
影响因子：
2.6
作者：
Bergmann M;Englert T;Stuetzer B;Hawley JR;Lappin MR;Hartmann K
通讯作者：
Hartmann K
影响因子：
3.1
作者：
Boulouis, HJ;Barrat, F;Chomel, BB
通讯作者：
Chomel, BB
影响因子：
1.8
作者：
Guptill, L;Slater, LN;HogenEsch, H
通讯作者：
HogenEsch, H