Design of a ratiometric two-photon probe for imaging of hypochlorous acid (HClO) in wounded tissues.
Design of a ratiometric two-photon probe for imaging of hypochlorous acid (HClO) in wounded tissues.
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用于次氯酸（HCLO）在受伤组织中成像的比率双光子探针的设计
DOI：
10.1039/c8sc01697f
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发表时间：
2018-07-28
期刊：
影响因子：
8.4
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Mao Z;Ye M;Hu W;Ye X;Wang Y;Zhang H;Li C;Liu Z
设计了一种比率双光子探针来可视化组织中的HClO水平，首次揭示了小鼠伤口修复过程中HClO的产生。HClO在广泛的生物和病理过程中发挥着重要作用。最近的研究表明，HClO的产生与伤口愈合过程密切相关。因此，建立一种可靠的检测创伤组织中HClO的方法具有重要意义。为此，我们在这里报道了一种合理设计的基于喹诺酮的比率计量型双光子荧光探针QClO，用于HClO。探针QClO由于氧硫杂环戊烷的氧化，响应于HClO迅速显示出蓝光发射的下降和绿光发射的增加。荧光强度比(绿/蓝)可以作为高灵敏度的HClO的比值检测信号。在确认其良好的体外传感性能后，通过检测活细胞中的外源性和内源性HClO对该探针进行了验证。该探针能够通过双光子显微镜对小鼠创伤组织中的HClO进行原位监测，从而显示了创伤愈合过程中HClO的生成情况。这项工作为HClO的检测和成像提供了一种简单可靠的工具，有望在HClO相关的生物学和病理学研究中获得更多的应用。
A ratiometric two-photon probe was designed to visualize HClO levels in tissues, revealing the generation of HClO in the wound-repairing process of mice for the first time. HClO plays crucial roles in a wide range of biological and pathological processes. Recent studies have revealed that the generation of HClO has close links with the wound healing process. It's thus meaningful to develop a reliable method for monitoring HClO in wounded tissues. Toward this purpose, we herein report a rationally designed quinolone-based ratiometric two-photon fluorescent probe, QClO, for HClO. The probe QClO rapidly displays a drop in blue emission and an increase of green emission in response to HClO due to the oxidation of oxathiolane. The fluorescence intensity ratio (green/blue) can serve as the ratiometric detection signal for HClO with high sensitivity. After confirming its excellent sensing performance in vitro, the probe was validated by detecting exogenous and endogenous HClO in living cells. The probe was capable of monitoring HClO in situ in the wounded tissues of mice by two-photon microscopy, which demonstrated the production profile of HClO during the wound-healing process. This work affords a simple and reliable tool for the detection and imaging of HClO, which promises to find more applications in HClO-related biological and pathological studies.
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DOI：
10.1038/nri3423
发表时间：
2013-05
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
8.4
作者：
Hu JJ;Wong NK;Lu MY;Chen X;Ye S;Zhao AQ;Gao P;Yi-Tsun Kao R;Shen J;Yang D
通讯作者：
Yang D
影响因子：
46.2
作者：
Lee MH;Kim JS;Sessler JL
通讯作者：
Sessler JL
通过双光子荧光探针选择性实时检测活细胞和组织切片中的一氧化氮
影响因子：
7.4
作者：
Dai, Chun-Guang;Wang, Ji-Long;Song, Qin-Hua
通讯作者：
Song, Qin-Hua
影响因子：
62.1
作者：
Kobayashi, Hisataka;Ogawa, Mikako;Alford, Raphael;Choyke, Peter L.;Urano, Yasuteru
通讯作者：
Urano, Yasuteru