A small diameter, fibrous vascular conduit generated from a poly(ester urethane)urea and phospholipid polymer blend.
A small diameter, fibrous vascular conduit generated from a poly(ester urethane)urea and phospholipid polymer blend.
复制标题
DOI：
10.1016/j.biomaterials.2009.01.013
复制
发表时间：
2009-05
期刊：
影响因子：
14
通讯作者：
Wagner, William R.
中科院分区：
文献类型：
--
作者：
Hong, Yi;Ye, Sang-Ho;Nieponice, Alejandro;Soletti, Lorenzo;Vorp, David A.;Wagner, William R.
与合成小直径血管移植物相关的血栓形成和增生限制已经产生了寻找原位自体血管段生成的组织工程解决方案的持续兴趣。一种方法是在血管组织发育的同时在该部位放置可生物降解的支架，以提供急性机械支持。为了产生具有适于血管组织的非血栓形成特性和机械特性的支架，将可生物降解的聚（酯氨基甲酸酯）脲（PEUU）和非血栓形成的生物激发磷脂聚合物，聚（2-甲基丙烯酰氧基乙基磷酰胆碱-共-甲基丙烯酰氧基乙基丁基氨基甲酸酯）（PMBU）以0-15%的PMBU重量分数共混，并静电纺丝以产生纤维支架。复合支架具有柔性，断裂应变超过300%，拉伸强度为7-10 MPa，顺应性为2.9-4.4 × 10 - 4 mmHg-1。随着PMBU含量的增加，支架表面的体外血小板沉积显著减少。大鼠平滑肌细胞增殖在PEUU/PMBU共混支架上也受到抑制，且PMBU含量越高，抑制作用越强。在大鼠腹主动脉中植入8周的纤维血管导管（内径1.3 mm）显示，与不含PMBU的PEUU相比，含15% PMBU的移植物具有更大的通畅性（67%对40%）。在PEUU/PMBU血管移植物中观察到薄的新生内膜层，内皮覆盖和良好的吻合组织整合。这些结果是令人鼓舞的进一步评价这种技术在更长的植入时间更大的直径应用。
The thrombotic and hyperplastic limitations associated with synthetic small diameter vascular grafts has generated sustained interest in finding a tissue engineering solution for autologous vascular segment generation in situ. One approach is to place a biodegradable scaffold at the site that would provide acute mechanical support while vascular tissue develops. To generate a scaffold that possessed both non-thrombogenic character and mechanical properties appropriate for vascular tissue, a biodegradable poly(ester urethane)urea (PEUU) and non-thromobogenic bioinspired phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-co-methacryloyloxyethyl butylurethane) (PMBU) were blended at PMBU weight fractions of 0–15% and electrospun to create fibrous scaffolds. The composite scaffolds were flexible with breaking strains exceeding 300%, tensile strengths of 7–10 MPa and compliances of 2.9–4.4 × 10−4 mmHg−1. In vitro platelet deposition on the scaffold surfaces significantly decreased with increasing PMBU content. Rat smooth muscle cell proliferation was also inhibited on PEUU/PMBU blended scaffolds with greater inhibition at higher PMBU content. Fibrous vascular conduits (1.3 mm inner diameter) implanted in the rat abdominal aorta for 8 weeks showed greater patency for grafts with 15% PMBU blending versus PEUU without PMBU (67% versus 40%). A thin neo-intimal layer with endothelial coverage and good anastomotic tissue integration was seen for the PEUU/PMBU vascular grafts. These results are encouraging for further evaluation of this technique in larger diameter applications for longer implant periods.
登录
查看更多内容
影响因子：
14
作者：
Hasegawa, T;Iwasaki, Y;Ishihara, K
通讯作者：
Ishihara, K
DOI：
10.1163/156856204323046906
发表时间：
2004-01-01
影响因子：
3.6
作者：
Jeong, SI;Kim, SH;Lee, YM
通讯作者：
Lee, YM
影响因子：
4.9
作者：
Khorasani, MT;Shorgashti, S
通讯作者：
Shorgashti, S
影响因子：
1.7
作者：
Kim, W. S.;Seo, J. W.;Kim, W. G.
通讯作者：
Kim, W. G.
影响因子：
4.9
作者：
Gao, Jin;Crapo, Peter;Wang, Yadong
通讯作者：
Wang, Yadong