HRPZyme Assisted Recognition of SARS-CoV-2 infection by Optical Measurement (HARIOM).
HRPZyme Assisted Recognition of SARS-CoV-2 infection by Optical Measurement (HARIOM).
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HRPZyme Assisted Recognition of SARS-CoV-2 Infection by Optical Measurement（HRPZyme Assisted Recognition of SARS-CoV-2 Infection by Optical Measurement）
DOI：
10.1016/j.bios.2021.113280
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发表时间：
2021-09-01
影响因子：
12.6
通讯作者：
Kulshreshtha A
中科院分区：
文献类型：
--
作者：
Ahmad M;Sharma P;Kamai A;Agrawal A;Faruq M;Kulshreshtha A
为了确定公共卫生政策，简单、廉价和可靠的严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）检测方法对于在资源有限的环境中进行大规模检测至关重要。目前用于鉴定SARS-CoV-2感染的分子方法的选择包括用于病毒遗传物质的基于核酸的检测（NAT）和用于病毒蛋白鉴定的基于抗原的检测。使用抗体检测试验检测宿主暴露。虽然NAT需要复杂的仪器和训练有素的人力，但抗原检测因其低灵敏度和特异性而受到困扰。因此，提供作为比色读数的感染存在的灵敏检测的测试有望实现由最低限度训练的人员在资源受限的环境中进行大规模测试。在这里，我们提出了一种新的HRPZyme辅助识别感染的光学测量（HARIOM）测定，结合特异性的NAT与灵敏度的酶测定，导致增强的信噪比在一个容易解释的比色读数。使用这种检测方法，我们可以检测到多达102个拷贝的合成病毒RNA掺入唾液作为检测基质。在疑似人类受试者上验证我们的测定，我们发现与基于PCR的读数一致，在不到一小时内阳性和阴性样品之间具有可见的比色区别。我们相信，这种检测方法具有帮助大规模筛查以检测SARS-CoV-2感染的潜力，通过促进比色检测，使用最少的资源和较少的训练有素的人员。
In order to define public health policies, simple, inexpensive and robust detection methods for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are vital for mass-testing in resource limited settings. The current choice of molecular methods for identification of SARS-CoV-2 infection includes nucleic acid-based testing (NAT) for viral genetic material and antigen-based testing for viral protein identification. Host exposure is detected using antibody detection assays. While NATs require sophisticated instrument and trained manpower, antigen tests are plagued by their low sensitivity and specificity. Thus, a test offering sensitive detection for presence of infection as a colorimetric readout holds promise to enable mass testing in resource constrained environments by minimally trained personnel. Here we present a novel HRPZyme Assisted Recognition of Infection by Optical Measurement (HARIOM) assay which combines specificity of NATs with sensitivity of enzymatic assays resulting in enhanced signal to noise ratios in an easily interpretable colorimetric readout. Using this assay, we could detect up to 102 copies of synthetic viral RNA spiked in saliva as a detection matrix. Validating our assay on suspected human subjects, we found concordance with PCR based readouts with visible colorimetric distinction between positive and negative samples in less than an hour. We believe that this assay holds the potential to aid in mass screening to detect SARS-CoV-2 infection by facilitating colorimetric detection with minimal resources and less trained personnel.
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