Charge variants in serum and urine hCG.
Charge variants in serum and urine hCG.
复制标题
血清和尿液 hCG 中的电荷变异。
DOI：
10.1016/j.cccn.2003.12.006
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Sutton,JaimeM
中科院分区：
文献类型：
--
作者：
Sutton,JaimeM
背景在美国销售的所有血清和尿液妊娠试验均根据WHO第3和第4国际标准（第3和第4 I.S.）人绒毛膜促性腺激素（hCG）。这些标准品已从妊娠尿液中分离出来;然而，它们用于校准，并产生尿液和血清hCG检测中使用的抗体。hCG分子的唾液酸含量可能不同;这改变了分子的酸性。已发表的研究表明，这些碳水化合物元素可能会改变不同血清和尿液hCG测试中hCG的识别。我们调查的电荷变体的hCG在血清和尿液样品中，并在hCG standards.Methodssamples进行了分析，通过制备等电聚焦。hCG的电荷变体进行定量使用DPC Immulite hCG assay.ResultsA的差异，观察到在尿液和血清样品中的电荷变体的比例。有一个显着更高的比例，更酸性的变体在尿液samples.ConclusionsUrine-derived标准可能不代表血清hCG，因此可能不适合校准血清检测。使用不同的免疫测定法时，hCG测试结果之间的变化多年来一直是一个持续存在的问题。需要进一步的研究，重点是尿和血清hCG的分子差异，以及其他因素，以确定其意义和贡献的问题时，比较hCG结果的测定间变异。
BackgroundAll serum and urine pregnancy tests sold in the United States are calibrated against the WHO 3rd and 4th International Standards (3rd and 4th I.S.) of Human Chorionic Gonadotropin (hCG). These standards have been isolated from pregnancy urine; however, they are used to calibrate, and generate antibodies used in both urine and serum hCG tests. hCG molecules may vary in sialic acid content; this changes the acidity of the molecule. Published studies have shown that these carbohydrate elements may alter recognition of hCG in different serum and urine hCG tests. We investigated the charge variants of hCG in serum and urine samples, and in hCG standards.MethodsSamples were analyzed by preparative isoelectric focusing. Charge variants of hCG were quantitated using the DPC Immulite hCG assay.ResultsA difference was observed in the proportion of charge variants in urine and serum samples. There was a significantly higher proportion of more-acidic variants in the urine samples.ConclusionsUrine-derived standards may not be representative of serum hCG and therefore may not be appropriate for calibrating serum assays. Variation among hCG test results when using different immunoassays has been a persistent problem for years. Additional studies are needed to focus on the molecular dissimilarity of urine and serum hCG, as well as other factors, to determine their significance and contribution to the problem of interassay variation when comparing hCG results.
登录
查看更多内容
影响因子：
2.8
作者：
Cole, LA;Khanlian, SA;Stephens, ND
通讯作者：
Stephens, ND
人绒毛膜促性腺激素及其亚基的新参比制剂。
影响因子：
11.1
作者：
R. Canfield;G. Ross
通讯作者：
G. Ross
定量测定血清人绒毛膜促性腺激素。
影响因子：
0.2
作者：
C. Smikle;K. Sorem;F. Wians;G. Hankins
通讯作者：
G. Hankins
肝半乳糖受体清除循环γ-谷氨酰转移酶。
DOI：
--
发表时间：
1993
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
N. Huseby;B. Mortensen;B. Smedsrød
通讯作者：
B. Smedsrød
人绒毛膜促性腺激素亚基的代谢清除率。
影响因子：
5.8
作者：
R. E. Wehmann;B. C. Nisula
通讯作者：
B. C. Nisula