Activation of phenotypically-distinct neuronal subpopulations of the rat amygdala following exposure to predator odor.
Activation of phenotypically-distinct neuronal subpopulations of the rat amygdala following exposure to predator odor.
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DOI：
10.1016/j.neuroscience.2010.12.001
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发表时间：
2011-02-23
期刊：
影响因子：
3.3
通讯作者：
Wilson, M. A.
中科院分区：
文献类型：
--
作者：
Butler, R. K.;Sharko, A. C.;Oliver, E. M.;Brito-Vargas, P.;Kaigler, K. F.;Fadel, J. R.;Wilson, M. A.
将老鼠暴露在捕食者的气味中会引发一种天生的恐惧反应。此外，已有研究表明，这种暴露会激活杏仁核等边缘脑区。然而，关于捕食者气味暴露后激活的杏仁核神经元的表型特征的数据很少。在目前的实验中，老鼠被暴露在含有雪貂气味、丁酸或没有气味的布料中30分钟。与对照组相比，暴露于雪貂气味中的大鼠表现出更多的防御掩埋。采用免疫组织化学双标记方法，在杏仁核基底外侧核(BLA)、中央核(CEA)和内侧核(MEA)进行c-Fos与钙/钙调素依赖的蛋白激酶II(CaMKII)、小蛋白或钙结合素(Calbindin)共定位计数。免疫组织化学双标结果显示，与对照组和丁酸暴露组相比，暴露于雪貂气味的大鼠的BLA、CEA和MEA中CaMKII阳性神经元的百分比显著增加。进一步的结果显示，与对照组和丁酸暴露组大鼠相比，雪貂气味暴露组大鼠大脑皮质前部Calbindin/c-Fos免疫反应阳性神经元显著减少，而与对照组和雪貂气味暴露组相比，MEA表达的Calbindin/c-Fos双标神经元显著减少。这些结果通过显示激活的杏仁核神经元的表型特征，增强了我们对暴露在捕食者威胁下的杏仁核功能的理解。有了这些知识，可以针对特定的神经元群体进一步阐明焦虑的基本基础，并可能为焦虑的治疗指明新的靶点。
Exposure of rats to an odor of a predator can elicit an innate fear response. In addition, such exposure has been shown to activate limbic brain regions such as the amygdala. However, there is a paucity of data on the phenotypic characteristics of the activated amygdalar neurons following predator odor exposure. In the current experiments, rats were exposed to cloth which contained either ferret odor, butyric acid, or no odor for 30 minutes. Ferret odor-exposed rats displayed an increase in defensive burying versus control rats. Sections of the brains were prepared for dual-labeled immunohistochemistry and counts of c-Fos co-localized with Ca2+/calmodulin-dependent protein kinase II (CAMKII), parvalbumin, or calbindin were made in the basolateral (BLA), central (CEA), and medial (MEA) nucleus of the amygdala. Dual-labeled immunohistochemistry showed a significant increase in the percentage of CAMKII–positive neurons also immunoreactive for c-Fos in the BLA, CEA and MEA of ferret odor-exposed rats compared to control and butyric acid-exposed groups. Further results showed a significant decrease in calbindin-immunoreactive neurons that were also c-Fos-positive in the anterior portion of the BLA of ferret odor-exposed rats compared to control and butyric acid-exposed rats, whereas the MEA expressed a significant decrease in calbindin/c-Fos dual-labeled neurons in butyric acid-exposed rats compared to controls and ferret odor-exposed groups. These results enhance our understanding of the functioning of the amygdala following exposure to predator threats by showing phenotypic characteristics of activated amygdalar neurons. With this knowledge, specific neuronal populations could be targeted to further elucidate the fundamental underpinnings of anxiety and could possibly indicate new targets for the therapeutic treatment of anxiety.
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影响因子：
3
作者：
BARDEN, N;MERAND, Y;DUPONT, A
通讯作者：
DUPONT, A
影响因子：
2.7
作者：
Masini, Cher V.;Garcia, Robert J.;Sasse, Sarah K.;Nyhuis, Tara J.;Day, Heidi E. W.;Campeau, Serge
通讯作者：
Campeau, Serge
影响因子：
3.6
作者：
BLANCHARD, RJ;BLANCHARD, DC;MEYER, S
通讯作者：
MEYER, S
影响因子：
3
作者：
FISCHMAN, AJ;MOLDOW, RL
通讯作者：
MOLDOW, RL
影响因子：
2.9
作者：
Masini, Cher V.;Sasse, Sarah K.;Campeau, Serge
通讯作者：
Campeau, Serge