Osteoconductive protamine-based polyelectrolyte multilayer functionalized surfaces.
Osteoconductive protamine-based polyelectrolyte multilayer functionalized surfaces.
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DOI：
10.1016/j.biomaterials.2011.06.032
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发表时间：
2011-10
期刊：
影响因子：
14
通讯作者：
Hammond, Paula T.
中科院分区：
文献类型：
--
作者：
Samuel, Raymond E.;Shukla, Anita;Paik, Daniel H.;Wang, Mary X.;Fang, Jean C.;Schmidt, Daniel J.;Hammond, Paula T.
关键词：
骨科植入物与宿主骨的整合是关节成形术、脊柱融合和肿瘤重建的主要挑战。细胞微环境可以通过植入体表面功能化来编程，允许在植入体-骨界面直接调节成骨细胞的粘附、增殖和分化。逐层组装聚电解质多层（PEM）结构的发展极大地扩展了我们制造复杂的纳米至微米级薄膜涂层的能力，这些薄膜涂层符合复杂的植入物几何形状。在许多生物医学应用中，薄PEM植入涂层的体内治疗效果已经被报道过。我们制备了基于蛋白蛋白的PEM薄膜，支持成骨前细胞在非交联膜涂层表面的长期增殖和分化。这些具有纳米级粗糙度的亲水性PEM功能化表面促进了体外成骨细胞钙化基质的沉积，从而提供了增强植入物与宿主骨整合的潜力。该涂层对干细胞的成骨培养和新治疗因子成骨潜能的评估具有直接影响。
The integration of orthopedic implants with host bone presents a major challenge in joint arthroplasty, spinal fusion and tumor reconstruction. The cellular microenvironment can be programmed via implant surface functionalization allowing direct modulation of osteoblast adhesion, proliferation, and differentiation at the implant-bone interface. The development of layer-by-layer assembled polyelectrolyte multilayer (PEM) architectures has greatly expanded our ability to fabricate intricate nanometer to micron scale thin film coatings that conform to complex implant geometries. The in vivo therapeutic efficacy of thin PEM implant coatings for numerous biomedical applications has previously been reported. We have fabricated protamine-based PEM thin films that support the long-term proliferation and differentiation of pre-osteoblast cells on non-cross-linked film coated surfaces. These hydrophilic PEM functionalized surfaces with nanometer-scale roughness facilitated increased deposition of calcified matrix by osteoblasts in vitro, and thus offer the potential to enhance implant integration with host bone. The coatings can make an immediate impact in the osteogenic culture of stem cells and assessment of the osteogenic potential of new therapeutic factors.
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影响因子：
4.9
作者：
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通讯作者：
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影响因子：
14
作者：
de Jonge, Lise T.;Leeuwenburgh, Sander C. G.;Jansen, John A.
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影响因子：
14
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.2
作者：
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通讯作者：
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