Metabolite Cross-Feeding between Rhodococcus ruber YYL and Bacillus cereus MLY1 in the Biodegradation of Tetrahydrofuran under pH Stress
Metabolite Cross-Feeding between Rhodococcus ruber YYL and Bacillus cereus MLY1 in the Biodegradation of Tetrahydrofuran under pH Stress
复制标题
pH 胁迫下四氢呋喃生物降解中红球菌 YYL 和蜡样芽胞杆菌 MLY1 代谢物交叉喂养
DOI：
10.1128/aem.01196-19
复制
发表时间：
2019-08
影响因子：
4.4
通讯作者：
Lu Zhenmei
中科院分区：
文献类型：
--
作者：
Liu Zubi;Huang Hui;Qi Minbo;Wang Xuejun;Adebanjo Omosalewa O;Lu Zhenmei
红球菌在不同的环境生态位中被发现，可以降解许多难降解的有毒污染物。然而，由于环境条件的复杂性和污染物降解微生物生长的限制，这些菌株的污染物降解效率严重降低。本研究中，蜡状芽孢杆菌MLY 1菌株表现出较强的抗逆性，能够适应各种环境，并通过代谢互食的方式提高了红红球菌YYL的THF降解效率，缓解了pH胁迫。这些研究结果表明，代谢物的交叉喂养发生在一个互补的方式，允许一个污染物降解菌株与非降解菌株在生物降解的各种柠檬酸类化合物。代谢交换的研究对于阐明降解菌和共生菌相互作用以在环境胁迫下生存的机制至关重要。摘要菌群是环境污染物生物降解的最基本单位之一。降解污染物的菌株经常遇到不同类型的环境压力，并且必须能够与污染环境中存在的其他细菌一起生存。在这项研究中，我们提出了一个非接触的相互作用模式之间的四氢呋喃（THF）降解菌株，红红球菌YYL，和非THF降解菌株，蜡状芽孢杆菌MLY 1。通过生理和分子生物学研究探讨了菌株YYL和MLY 1之间的代谢相互作用机制，并通过基于液相色谱-质谱的代谢组学分析，进一步得到了菌株YYL在最适pH（pH 8.3）和pH胁迫（pH 7.0）下的代谢响应曲线的支持。结果表明，共培养系统通过3种途径抵抗pH胁迫：（1）菌株MLY 1利用酸性代谢产物，影响谷氨酰胺的比例，导致细胞内pH升高;（2）菌株MLY 1具有降解中间产物的能力，缓解了菌株YYL的产物抑制;菌株MLY 1产生的微量营养物质是YYL在pH胁迫下生长的必需物质，而菌株YYL产生的THF降解中间产物是菌株MLY 1的主要营养物质。此外，代谢物交叉喂养相互作用方面的污染物的生物降解进行了讨论。重要性红球菌属已被发现在不同的环境小生境，并能降解许多有毒污染物。然而，由于环境条件的复杂性和污染物降解微生物生长的限制，这些菌株的污染物降解效率严重降低。本研究中，蜡状芽孢杆菌MLY 1菌株表现出较强的抗逆性，能够适应各种环境，并通过代谢互食的方式提高了红红球菌YYL的THF降解效率，缓解了pH胁迫。这些研究结果表明，代谢物的交叉喂养发生在一个互补的方式，允许一个污染物降解菌株与非降解菌株在生物降解的各种柠檬酸类化合物。代谢交换的研究对于阐明降解菌和共生菌相互作用以在环境胁迫下生存的机制至关重要。
Rhodococcus species have been discovered in diverse environmental niches and can degrade numerous recalcitrant toxic pollutants. However, the pollutant degradation efficiency of these strains is severely reduced due to the complexity of environmental conditions and limitations in the growth of the pollutant-degrading microorganism. In our study, Bacillus cereus strain MLY1 exhibited strong stress resistance to adapt to various environments and improved the THF degradation efficiency of Rhodococcus ruber YYL by a metabolic cross-feeding interaction style to relieve the pH stress. These findings suggest that metabolite cross-feeding occurred in a complementary manner, allowing a pollutant-degrading strain to collaborate with a nondegrading strain in the biodegradation of various recalcitrant compounds. The study of metabolic exchanges is crucial to elucidate mechanisms by which degrading and symbiotic bacteria interact to survive environmental stress. ABSTRACT Bacterial consortia are among the most basic units in the biodegradation of environmental pollutants. Pollutant-degrading strains frequently encounter different types of environmental stresses and must be able to survive with other bacteria present in the polluted environments. In this study, we proposed a noncontact interaction mode between a tetrahydrofuran (THF)-degrading strain, Rhodococcus ruber YYL, and a non-THF-degrading strain, Bacillus cereus MLY1. The metabolic interaction mechanism between strains YYL and MLY1 was explored through physiological and molecular studies and was further supported by the metabolic response profile of strain YYL, both monocultured and cocultured with strain MLY1 at the optimal pH (pH 8.3) and under pH stress (pH 7.0), through a liquid chromatography-mass spectrometry-based metabolomic analysis. The results suggested that the coculture system resists pH stress in three ways: (i) strain MLY1 utilized acid metabolites and impacted the proportion of glutamine, resulting in an elevated intracellular pH of the system; (ii) strain MLY1 had the ability to degrade intermediates, thus alleviating the product inhibition of strain YYL; and (iii) strain MLY1 produced some essential micronutrients for strain YYL to aid the growth of this strain under pH stress, while strain YYL produced THF degradation intermediates for strain MLY1 as major nutrients. In addition, a metabolite cross-feeding interaction with respect to pollutant biodegradation is discussed. IMPORTANCE Rhodococcus species have been discovered in diverse environmental niches and can degrade numerous recalcitrant toxic pollutants. However, the pollutant degradation efficiency of these strains is severely reduced due to the complexity of environmental conditions and limitations in the growth of the pollutant-degrading microorganism. In our study, Bacillus cereus strain MLY1 exhibited strong stress resistance to adapt to various environments and improved the THF degradation efficiency of Rhodococcus ruber YYL by a metabolic cross-feeding interaction style to relieve the pH stress. These findings suggest that metabolite cross-feeding occurred in a complementary manner, allowing a pollutant-degrading strain to collaborate with a nondegrading strain in the biodegradation of various recalcitrant compounds. The study of metabolic exchanges is crucial to elucidate mechanisms by which degrading and symbiotic bacteria interact to survive environmental stress.
登录
查看更多内容
影响因子：
13.6
作者：
Xue-ling Wu;R. Liang;Qin-yun Dai;D. Jin;Yangyang Wang;W. Chao
通讯作者：
Xue-ling Wu;R. Liang;Qin-yun Dai;D. Jin;Yangyang Wang;W. Chao
影响因子：
1.5
作者：
S. Sure;L. Ackland;A. Torriero;A. Adholeya;Mandira Kochar
通讯作者：
S. Sure;L. Ackland;A. Torriero;A. Adholeya;Mandira Kochar
影响因子：
6.8
作者：
Chuanyuan Wang;Xing Liu;Jie Guo;Yingchun Lv;Yuanwei Li
通讯作者：
Chuanyuan Wang;Xing Liu;Jie Guo;Yingchun Lv;Yuanwei Li
影响因子：
16.6
作者：
Benomar, Saida;Ranava, David;Giudici-Orticoni, Marie-Therese
通讯作者：
Giudici-Orticoni, Marie-Therese
影响因子：
11.4
作者：
Yanlai Yao;Z. Lv;H. Min;Zhenhua Lv;Huipeng Jiao
通讯作者：
Yanlai Yao;Z. Lv;H. Min;Zhenhua Lv;Huipeng Jiao